Changes of blood brain barrier permeability after experimental subarachnoid hemorrhage

谢宗义; 马颖; 程远

您当前的位置：

首页 >

文章列表页 >

Changes of blood brain barrier permeability after experimental subarachnoid hemorrhage

更新时间：2026-01-09

- Changes of blood brain barrier permeability after experimental subarachnoid hemorrhage
- Journal of Army Medical University Vol. 29, Issue 9, Pages: 834-836(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R741.02;R743.35
- Published：2007
- 稿件说明：
移动端阅览
谢宗义,马颖,程远.大鼠蛛网膜下腔出血后血脑屏障通透性变化[J].陆军军医大学学报(原第三军医大学学报),2007,29(09):834-836.

　XIE Zong-yi,MA Ying,CHENG Yuan.Changes of blood brain barrier permeability after experimental subarachnoid hemorrhage[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(09):834-836.
谢宗义,马颖,程远.大鼠蛛网膜下腔出血后血脑屏障通透性变化[J].陆军军医大学学报(原第三军医大学学报),2007,29(09):834-836. DOI：

　XIE Zong-yi,MA Ying,CHENG Yuan.Changes of blood brain barrier permeability after experimental subarachnoid hemorrhage[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(09):834-836. DOI：

摘要

目的探讨大鼠蛛网膜下腔出血(subarachnoid hemorrhage

SAH)后血脑屏障（blood brain barrier，BBB）通透性变化。方法雌性SD大鼠80只，分为生理盐水对照组（NS组）和SAH组。采用经额叶底面蛛网膜下腔插管至Willis环注血法建立SAH模型，在SAH后6、12、24、36、48、60、72 h等不同时间点，应用荧光分光光度计和荧光显微镜方法分别检测大脑皮质BBB对伊文思蓝(Evans Blue

EB)的通透性，同时利用透射电镜观察BBB超微结构变化。结果与NS组比较，大脑皮质EB含量和漏出量的最大值均出现在SAH后36 h时（P0.05），于60 h时恢复至正常水平（P0.05）。BBB超微结构改变在SAH后36 h时最明显。结论 BBB通透性改变发生在SAH急性期，是由多种因素共同所致，而且SAH后BBB具有自我修复特性。

Abstract

Objective To explore the dynamic changes of blood brain barrier (BBB) permeability after experimental subarachnoid hemorrhage (SAH) in rats. Methods Eighty female SD rats were divided into normal saline control group (n=10) and SAH group (n=70). The SAH model was induced by injecting 300 μl of autologous arterial blood into the subarachnoid space near the circle of Willis via a cannula in an artificial hole between the olfactory bulb and frontal lobe. BBB permeability in cerebral cortex of SAH and normal saline rats was assessed at 6

and 72 h after SAH establishment by fluorescence spectrophotometer and fluorescence microscopy for Evans Blue (EB) extravasation. The ultrastructural changes in BBB were observed with transmission electron microscope. Results Compared with the control group

the changes of Evans Blue content and extravasation in cerebral cortex of SAH group peaked at 36 h (P0.05)

and down to the normal level at 60 h after SAH (P0.05). The severe ultrastructural abnormality was found at 36 h after SAH. Conclusion The changes of BBB permeability develop at the acute stage of SAH

resulting from multiple factors together. The BBB after SAH possesses a self-repairable property.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Iron ion-induced acute brain injury in rats with intraventricular hemorrhage

Expression profile of catechol-O-methyltransferase in rats after subarachnoid hemorrhage

Permeability of blood-brain barrier by ultrasound-induced microbubble destruction or mannitol osmotic opening in rats

Early identification of delayed cerebral ischemia following aneurysmal subarachnoid hemorrhage: development and validation of a multifactorial clinical prediction model

Activation of PDGFR and ROS in early brain injury in rats after subarachnoid hemorrhage

Related Author

张建波

唐俊

杨力铭

陈前伟

郭靖

陈志

朱刚

何朝晖

Related Institution

Department of Neurosurgery， the Second Affiliated Hospital of Chongqing Medical University

Department of Neurosurgery, the First Affiliated Hospital of Chongqing Medical University

Department of Neurosurgery, Changshou Distict People's Hospital

Department of Neurosurgery, Yongchuan Hospital, Chongqing Medical University

Department of Critical Care Medicine, Yongchuan Hospital, Chongqing Medical University

⁰