Effect of artemisinin on lung injury in septic rats

曹红卫; 郭毅斌; 魏利召; 王宁; 曲海; 周红; 郑江

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Effect of artemisinin on lung injury in septic rats

更新时间：2026-01-09

- Effect of artemisinin on lung injury in septic rats
- Journal of Army Medical University Vol. 29, Issue 10, Pages: 951-954(2007)
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  CLC： R282.71;R563.05;R631
- Published：2007
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曹红卫,郭毅斌,魏利召,王宁,曲海,周红,郑江.青蒿素对脓毒症大鼠肺损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):951-954.

　CAO Hong-wei,GUO Yi-bin,WEI Li-zhao,WANG Ning,QU Hai,ZHOU Hong,ZHENG Jiang.Effect of artemisinin on lung injury in septic rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):951-954.
曹红卫,郭毅斌,魏利召,王宁,曲海,周红,郑江.青蒿素对脓毒症大鼠肺损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):951-954. DOI：

　CAO Hong-wei,GUO Yi-bin,WEI Li-zhao,WANG Ning,QU Hai,ZHOU Hong,ZHENG Jiang.Effect of artemisinin on lung injury in septic rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):951-954. DOI：

摘要

目的观察青蒿素(artemisinin， ART)对脓毒症大鼠肺组织损伤的影响。方法采用盲肠结扎穿孔术（cecal ligation and puncture， CLP）制作大鼠脓毒症模型，动物分为正常对照组、CLP组和青蒿素治疗(ART)组，分别于CLP术后2、6、12、24、48、72 h活杀大鼠取肺组织，匀浆后动态浊度鲎试验法检测内毒素（lipopolysaccharide， LPS）水平，ELISA法检测TNF-α和IL-6的含量，实时荧光定量反转录聚合酶链反应（real-time RT-PCR）检测肺组织TNF-α和IL-6 mRNA的表达，光镜下观察肺组织的病理改变。结果 CLP术后，ART组大鼠肺组织匀浆LPS、TNF-α和IL-6含量较CLP组明显降低（P＜0.05，P＜0.01），TNF-α和IL-6 mRNA的表达也显著低于CLP组（P＜0.05，P＜0.01），24 h肺组织的病理损伤明显减轻。结论青蒿素可能通过降低脓毒症大鼠肺组织局部的LPS水平，抑制和减少了TNF-α和IL-6等促炎细胞因子的表达和释放，进而减轻肺组织的损伤。

Abstract

Objective To observe the effect of artemisinin (ART) on lung injury in septic rats. Methods Wistar rats were divided into control

cecal ligation and puncture (CLP)

intragastric administration of ART groups. Sepsis was induced by CLP. At 2

48 and 72 h after CLP

the lung homogenate was collected to examine the concentration of LPS by kinetic turbidimetric limulus test

cytokines of TNF-α and IL-6 by ELISA. Meanwhile

the total mRNA in lung tissues was extracted to detect the expression of TNF-α and IL-6 mRNA by real-time RT-PCR and the histomorphology of lung tissues was observed by light microscope. Results In ART group

the concentration of LPS

TNF-α and IL-6 in lung homogenate were significantly decreased than those in CLP group (P＜0.05 or P＜0.01) and the expressions of TNF-α and IL-6 mRNA were distinctly lower (P＜0.05 or P＜0.01). In addition

pathological injury of lung at 24 h was detectably improved after treated with ART. Conclusion ART might decrease LPS concentration

inhibit the expression and the releasing of proinflammatory cytokines in lung tissues so as to alleviate the pathological lung injury in septic rats.

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