Objective      To screen the proteins interacting with AMP-activated protein kinase α2 (AMPKα2) in rat brain cDNA library by bacterial two-hybrid system

and to investigate the biological role and the regulatory mechanism of AMPKα2 in brain.       Methods      The recombinant pBT-AMPKα2 was used as the bait to screen a rat fetal brain cDNA library by bacterial two-hybrid system. The plasmids of positive colonies were extracted and analyzed by DNA sequencing and BLAST search in GenBank.       Results      Seven proteins binding to AMPKα2 were screened out

including polyubiquitin

small heat shock protein 8

phosphofructokinase

cytochrome C oxidase subunit Ⅰ（COXⅠ）

HLA-B-associated transcript 3 (BAT3) isoform 1

protein tyrosine phosphatase receptor type D (Ptprd) and islet-brain 1 (IB1).       Conclusion      Polyubiquitin

  HSP8

phosphofructokinase

  COXⅠ

BAT3 isoform 1

Ptprd and IB1 can interact with AMPKα2 in brain.