Impact of abdominal cavity bacterial infection on immunological rejection following rat liver transplantation

丁隆; 杨宇; 董家鸿

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Impact of abdominal cavity bacterial infection on immunological rejection following rat liver transplantation

更新时间：2026-01-09

- Impact of abdominal cavity bacterial infection on immunological rejection following rat liver transplantation
- Journal of Army Medical University Vol. 29, Issue 11, Pages: 1072-1075(2007)
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  CLC： R392.4;R656;R657.3
- Published：2007
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丁隆,杨宇,董家鸿.大鼠肝移植术后腹腔细菌感染对移植肝免疫排斥的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1072-1075.

　DING Long,YANG Yu,DONG Jia-hong.Impact of abdominal cavity bacterial infection on immunological rejection following rat liver transplantation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1072-1075.
丁隆,杨宇,董家鸿.大鼠肝移植术后腹腔细菌感染对移植肝免疫排斥的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1072-1075. DOI：

　DING Long,YANG Yu,DONG Jia-hong.Impact of abdominal cavity bacterial infection on immunological rejection following rat liver transplantation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1072-1075. DOI：

摘要

目的探讨大鼠肝移植后腹腔细菌感染机体免疫状态的变化及对移植肝免疫排斥的作用。方法采用改良的Kamada双袖套法建立DA→LEW的肝移植模型，术后将大鼠分为3组：免疫排斥组（G1）

术后3 d腹腔生理盐水注射；移植后感染组（G2）

术后3 d给予腹腔大肠杆菌注射；免疫抑制组（G3）

术后常规给予免疫抑制药物(CsA，3 mg·kg

-1

·d

-1

)。感染后7 d处死动物，每组6个样本，通过流式细胞术测定外周淋巴细胞亚群，单向混合淋巴细胞培养检测淋巴细胞功能，RT-PCR检测肝内IL-4和IFN-γ mRNA的表达来评估机体的免疫状态的变化，肝病理切片观察对免疫排斥的影响。结果感染后7 d，G1、G2、G3组淋巴细胞数、CD4/CD8值下降[分别为(1.753±0.181)、(1.384±0.073)、（0.997±0.025）

]

，淋巴细胞功能降低[SI分别为（67.59±3.40）、(37.14±0.90)、（15.87±0.51）

]

，IL-4 mRNA表达增高，与G1组比较，G2组上述指标差异有统计学意义（P0.05）。与G3组相比，G2组虽然也有差异（P0.01），但从病理学切片观察，在一定程度上减轻了免疫排斥反应。结论肝移植术后腹腔细菌感染促进了Th2细胞的分化，下调CD4/CD8值并降低了淋巴细胞功能，部分减轻了肝移植术后急性排斥反应。

Abstract

Objective To investigate the changes of immune state and the impact on immunological rejection elicited by abdominal cavity bacterial infection after DA-Lewis rat liver transplantation. Methods Orthotopic liver transplant model was established by modified Kamada two-cuff technique. The animals were divided randomly into Group 1

isotonic Na chloride injected into abdominal cavity 3 days after operation; Group 2

mixed Bacillus coli

liquid injected instead of saline; Group 3

immunosuppressive drug CsA administered routinely after operation (3 mg·kg

-1

·d

-1

). All the animals were sacrificed 7 days after infection. The blood and graft samples were collected for cell-subpopulation

mixed lymphocyte culture

IL-4

IFN-γ mRNA detection and histological evaluation. Results Seven days after infection

the lympholeukocyte population

CD4/CD8（G1=1.753±0.181，G2=1.384±0.073，G3=0.997±0.025）and lympholeukocyte function（SI：G1=67.59±3.40，G2=37.14±0.90，G3=15.87±0.51）declined in Group 2 as compared with other groups and cellular differentiation drifted to Th2. There was significant difference between Group 2 and Group 1 or 3. Conclusion Abdominal cavity bacterial infection after rat liver transplantation will promote the differentiation of T cells into Th2

down-regulate CD4/CD8 ratio and immune function of lymphocytes and accordingly alleviate partly the acute rejection following liver transplantation.

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