Silencing breast cancer cell CXCR4 gene in vitro

厉红元; 任国胜; 谭金详

您当前的位置：

首页 >

文章列表页 >

Silencing breast cancer cell CXCR4 gene in vitro

更新时间：2026-01-09

- Silencing breast cancer cell CXCR4 gene in vitro
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 728-730(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394-33;R73-354;R737.9
- Published：2007
- 稿件说明：
移动端阅览
厉红元,任国胜,谭金详.siRNA沉默人乳腺癌细胞株CXCR4基因的实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):728-730.

　LI Hong-yuan,REN Guo-sheng,TAN Jin-xiang.Silencing breast cancer cell CXCR4 gene in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):728-730.
厉红元,任国胜,谭金详.siRNA沉默人乳腺癌细胞株CXCR4基因的实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):728-730. DOI：

　LI Hong-yuan,REN Guo-sheng,TAN Jin-xiang.Silencing breast cancer cell CXCR4 gene in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):728-730. DOI：

摘要

目的探讨shRNA- CXCR4对人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435s细胞CXCR4基因表达及蛋白表达的影响。方法通过shRNA-CXCR4沉默CXCR4基因后，RT-PCR、Western blotting检测3株人乳腺癌细胞MCF-7、MDA-MB-231、MDA-MB-435s的CXCR4 mRNA及其蛋白的表达。结果 shRNA-CXCR4作用于人乳腺癌细胞株MCF-7、MDA-MB-231、MDA-MB-435s后能明显抑制其CXCR4基因的mRNA及蛋白表达水平（P0.05）。结论 shRNA-CXCR4能特异性抑制乳腺癌细胞的CXCR4 mRNA及其蛋白的表达。

Abstract

Objective To study whether shRNA-CXCR4 could inhibit the CXCR4 gene expression in MCF-7

MDA-MB-231 and MDA-MB-435s breast cancer cells. Methods After the knockdown of CXCR4 by shRNA

the CXCR4 mRNA expression by RT-PCR and the CXCR4 protein expression by Western blotting in breast cancer cells were examined. Results The CXCR4 mRNA expression and its protein expression were decreased significantly in MCF-7

MDA-MB-231 and MDA-MB-435s breast cancer cells by shRNA-CXCR4（P0.05）. Conclusion Silencing CXCR4 could inhibit the breast cancer cell CXCR4 mRNA and its protein expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

A novel estrogen receptor expression stratification (low/moderate/high) based on Chinese population provides more accurate prognosis prediction for HR⁺/HER2^- early breast cancer patients

Sulfasalazine antagonizes boosted metastasis of breast cancer induced by ionizing radiation

Effect of transmembrane protein 196 on migration and invasion of breast cancer

Effect of circular RNA hsa_circ_0000231 interacting with HnRNPK on proliferation, migration, and apoptosis in breast cancer

Circular RNA circACTN4 promotes proliferation, invasion and metastasis of breast cancer cells

Related Author

WANG Chengfang

ZHOU Yuqin

ZHANG Yanling

QI Xiaowei

ZHANG Yi

Ge HE

Guojian WANG

Na ZHAO

Related Institution

Department of Breast and Thyroid Surgery， First Affiliated Hospital， Army Medical University （Third Military Medical University）

State Key Laboratory of Trauma, Burns and Combined Injury, Department of Anti-Radiation Medicine, Chongqing Engineering Research Center for Nanomedicine, Institute of Combined Injury, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Department of Breast and Thyroid Surgery, the Second Affiliated Hospital of Chongqing Medical University

Department of Environmental Health, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Institute of Toxicology, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

⁰