Combination of sodium butyrate and cisplatin has antiproliferative effects on non-small lung cancer cell line

高娟; 饶进军; 吴少瑜; 文晓芸; 吕琳; 徐伟; 吴曙光

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Combination of sodium butyrate and cisplatin has antiproliferative effects on non-small lung cancer cell line

更新时间：2026-01-09

- Combination of sodium butyrate and cisplatin has antiproliferative effects on non-small lung cancer cell line
- Journal of Army Medical University Vol. 29, Issue 11, Pages: 1066-1068(2007)
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  CLC： R329.28;R734.2;R979.1
- Published：2007
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高娟,饶进军,吴少瑜,文晓芸,吕琳,徐伟,吴曙光.丁酸钠与顺铂联用对非小细胞性肺癌细胞生长的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1066-1068.

　GAO Juan,RAO Jin-jun,WU Shao-yu,WEN Xiao-yun,LU Lin,XU Wei,WU Shu-guang.Combination of sodium butyrate and cisplatin has antiproliferative effects on non-small lung cancer cell line[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1066-1068.
高娟,饶进军,吴少瑜,文晓芸,吕琳,徐伟,吴曙光.丁酸钠与顺铂联用对非小细胞性肺癌细胞生长的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1066-1068. DOI：

　GAO Juan,RAO Jin-jun,WU Shao-yu,WEN Xiao-yun,LU Lin,XU Wei,WU Shu-guang.Combination of sodium butyrate and cisplatin has antiproliferative effects on non-small lung cancer cell line[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1066-1068. DOI：

摘要

目的研究丁酸钠和顺铂联合作用对非小细胞性肺癌A549细胞增殖的影响及作用机制。方法采用CCK-8细胞计数法测定丁酸钠、顺铂联用对A549细胞增殖活性的影响，流式细胞法检测细胞周期和细胞凋亡的改变，RT-PCR法检测p21基因的表达。结果丁酸钠、顺铂联用可抑制A549细胞增殖且呈剂量依赖性，联用后的IC

降低至5.44 μmol/L，与单用DDP的IC

(10.25 μmol/L)相比降低了46.93％(P0.01)。联用可使细胞周期阻滞于G

期，同时诱导细胞凋亡和p21基因表达的上调。结论丁酸钠与顺铂联用能抑制A549细胞生长，其作用机制与促进细胞凋亡及诱导p21基因表达有关。

Abstract

Objective To investigate the antiproliferative effect of combination of sodium butyrate (NaB) and cisplatin (DDP) on non-small cell lung cancer cell line A549 and its possible mechanism. Methods The antiproliferative effects of combination NaB and DDP were evaluated using Cell Counting Kit-8 (CCK-8) assay. The changes of the cell cycle and the apoptosis were observed by flow cytometry. The mRNA expression level of p21 gene was detected by RT-PCR. Results The combination of NaB and DDP could inhibit the growth of A549 cell line in a dose and time dependent manner. The IC

of the combination group was 5.44 μmol/L and the IC

of DDP group was 10.25 μmol/

L. The combination of NaB and DDP induced cell cycle arrest at G

phase

and induced cell apoptosis and up-regulation of mRNA expression of p21 gene. Conclusion The combination of NaB and DDP has antiproliferative effects on non-small lung cancer cells

and the mechanism may be related to promoting cell apoptosis and inducing the expression of p21 gene.

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