Effects of glial cell line-derived neurotrophic factor gene-activated matrix on repair of sciatic nerve defect of rats

梁安霖; 蒋电明; 安洪

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Effects of glial cell line-derived neurotrophic factor gene-activated matrix on repair of sciatic nerve defect of rats

更新时间：2026-01-09

- Effects of glial cell line-derived neurotrophic factor gene-activated matrix on repair of sciatic nerve defect of rats
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 717-720(2007)
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  CLC： R318.08;R622.3;R651.3
- Published：2007
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梁安霖,蒋电明,安洪.GDNF基因活化的细胞外基质对大鼠坐骨神经缺损的修复作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):717-720.

　LIANG An-lin,JIANG Dian-ming,AN Hong.Effects of glial cell line-derived neurotrophic factor gene-activated matrix on repair of sciatic nerve defect of rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):717-720.
梁安霖,蒋电明,安洪.GDNF基因活化的细胞外基质对大鼠坐骨神经缺损的修复作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):717-720. DOI：

　LIANG An-lin,JIANG Dian-ming,AN Hong.Effects of glial cell line-derived neurotrophic factor gene-activated matrix on repair of sciatic nerve defect of rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):717-720. DOI：

摘要

目的探讨GDNF基因活化的细胞外基质对神经元、再生轴突和靶肌肉的保护作用。方法通过化学萃取获得大鼠坐骨神经细胞外基质，复合上编码GDNF的质粒DNA，构建成基因活化的细胞外基质支架。90只成年Wistar大鼠按随机数字法分为3组：GDNF基因活化的细胞外基质桥接组（A组，n=30)

ECM桥接组（B组，n=30)

自体神经桥接组（C组，n=30)，12周时用激光共聚焦显微镜等形态学方法及电生理学等方法来评价再生神经功能。结果 GDNF基因活化的细胞外基质能作为局部的基因缓释系统来释放GDNF，高效表达12周以上；再生轴突数达（2 117±294），坐骨神经指数恢复到（30.92±2.98）%。结论 GDNF基因活化的细胞外基质可以促进大鼠坐骨神经功能的恢复，有希望成为自体神经移植的替代品。

Abstract

Objective To evaluate the effects of glial cell line-derived neurotrophic factor (GDNF) gene-activated matrix (GAM) in bridging rat sciatic nerve defect. Methods The peripheral nerve extracellular matrix was harvested by chemical extraction. GDNF gene-activated matrix was comprised of the extracellular matrix and plasmids encoding GDNF. Ninety adult Wistar rats were randomly divided into three groups: group A (GDNF GAM conduits)

group B ( extracellular matrix conduits)

group C (autografts). At the end of 12 weeks

rats from each group were subjected to walking tract analysis

electrophysiological and histomorphological studies. Results GDNF DNA could be retained in GAM

promoting transgene expression in the sciatic nerve

in GAM

and more importantly

in axotomized neurons in spinal cord for 12 weeks. The number of regenerated axons for the GAM grafts in the distal sciatic nerve was lower than that for autografts (2 117±294 vs 2 474±297) at 12 weeks. The sciatic nerve function index for the GAM grafts was lower than that for autografts [(30.92±2.98)% vs (26.27±2.71) %] at 12 weeks. Conclusion GDNF GAM may be a promising alternative to autografts for repair of rat sciatic nerve.

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