Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells

张国桥; 凌贤龙; 陈正堂

您当前的位置：

首页 >

文章列表页 >

Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells

更新时间：2026-01-09

- Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 711-713(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.25;R73-3;R735.7
- Published：2007
- 稿件说明：
移动端阅览
张国桥,凌贤龙,陈正堂.线粒体DNA缺失肝癌细胞内活性氧、线粒体膜电位变化实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):711-713.

　ZHANG Guo-qiao,LING Xian-long,CHEN Zheng-tang.Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):711-713.
张国桥,凌贤龙,陈正堂.线粒体DNA缺失肝癌细胞内活性氧、线粒体膜电位变化实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):711-713. DOI：

　ZHANG Guo-qiao,LING Xian-long,CHEN Zheng-tang.Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):711-713. DOI：

摘要

目的比较活性氧、线粒体膜电位在线粒体DNA缺失肝癌细胞株SK-Hep1（ρ

SK-Hep1）与亲本细胞中的差别。 方法采用溴化乙锭诱导制备ρ

SK-Hep1细胞株；荧光探针标记、流式细胞计数及激光共聚焦显微镜方法检测细胞内活性氧和线粒体膜电位。结果 ρ

SK-Hep1细胞内活性氧荧光强度显著高于其亲本SK-Hep1细胞株(P0.01)，ρ

SK-Hep1肝癌细胞平均计数35.5，而SK-Hep1肝癌细胞平均计数15.9。ρ

SK-Hep1细胞线粒体膜电位显著低于亲本细胞(P0.01)，ρ

SK-Hep1细胞平均计数55.0，而SK-Hep1细胞平均计数65.9。结论线粒体DNA缺失后细胞内活性氧水平增加，而线粒体膜电位有所减低。

Abstract

Objective To observe the differences of the reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) in mitochondrial DNA-depleted SK-Hep1 and its parent cells. Methods ρ

SK-Hep1 cell line was generated by treating SK-Hep1 with ethidium bromide. Flow cytometry (FCM) and laser scanning confocal microscopy were used to detect ROS and ΔΨm. Results Fluorescence intensity of ROS in ρ

SK-Hep1 cells was significantly higher than that of the parent cells (35.5 vs 15.6)

and the ΔΨm was lower in ρ

SK-Hep1 cells (55.0 vs 65.9). Conclusion ρ

SK-Hep1 cells sho

wed elevated ROS and lower ΔΨm. These changes might contribute to the variation of phenotypes.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Apoptosis in mtDNA-depleted cells and its transmitochondrial cybrids

RANKL通过ROS介导HIF-1α下调促进自噬和破骨细胞分化

Preparation of reactive oxygen species scavenging Ce6-Luminol encapsulated liposomes and their protective effect against radiation pneumonitis in mice

Preparation of novel luminescent nanomicelles responsive to neutrophils and their imaging application in mouse burn model

Tetramethylpyrazine inhibits NETs formation induced by high glucose by regulating NRF2/HO-1 pathway

Related Author

李歆强

凌贤龙

周源

何玉琦

李诗伟

晏斌

LI Songtao

SUN Jing

Related Institution

Department of Nuclear Medicine， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Pharmaceutical Analytical Chemistry

Department of Pharmaceutics， Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

College of Bioengineering， Chongqing University

⁰