Isolation and enrichment of fetal nucleated red blood cells by using N-acetylgalactosamine-specific lectin

彭佳; 府伟灵; 黄庆; 王珏; 陈鸣; 黄君富

您当前的位置：

首页 >

文章列表页 >

Isolation and enrichment of fetal nucleated red blood cells by using N-acetylgalactosamine-specific lectin

更新时间：2026-01-09

- Isolation and enrichment of fetal nucleated red blood cells by using N-acetylgalactosamine-specific lectin
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 708-710(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R331.141;R343.23;R446.113
- Published：2007
- 稿件说明：
移动端阅览
彭佳,府伟灵,黄庆,王珏,陈鸣,黄君富.N-乙酰半乳糖特异性植物凝素分离和富集胎儿有核红细胞[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):708-710.

　PENG Jia,FU Wei-ling,HUANG Qing,WANG Jue,CHEN Ming,HUANG Jun-fu.Isolation and enrichment of fetal nucleated red blood cells by using N-acetylgalactosamine-specific lectin[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):708-710.
彭佳,府伟灵,黄庆,王珏,陈鸣,黄君富.N-乙酰半乳糖特异性植物凝素分离和富集胎儿有核红细胞[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):708-710. DOI：

　PENG Jia,FU Wei-ling,HUANG Qing,WANG Jue,CHEN Ming,HUANG Jun-fu.Isolation and enrichment of fetal nucleated red blood cells by using N-acetylgalactosamine-specific lectin[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):708-710. DOI：

摘要

目的采用N-乙酰半乳糖（N-Acetyl-D-galactosamine

GalNAc）包被的玻片分离和富集胎儿有核红细胞（fetal nucleated red blood cells，FNRBCs）。方法用密度梯度离心法收集胎儿脐血中的FNRBCs，然后将其与不同浓度的大豆凝聚素（SBA）混合，所形成的“FNRBCs-SBA”复合物用GalNAc包被的玻片来分离和富集，分析SBA工作浓度对FNRBCs分离和富集效率的影响。结果当GalNAc包被玻片的浓度为100 μg/ml时，20 μg/ml 的SBA浓度是分离和富集FNRBCs的最适浓度；此时WBC与有核红细胞的比值较低（27.7%），而FNRBCs与有核红细胞的比值最高（3.7%）。结论 N-乙酰半乳糖特异性植物凝素可用于选择性地分离和富集FNRBCs，与传统方法相比较具有无需特殊仪器与设备，简便快速的优点。

Abstract

Objective To isolate and enrich fetal nucleated red blood cells (FNRBCs). Methods Fetal cells were collected by gradient centrifugation from umbilical cord blood collected during delivery

then mixed with soybean agglutinin (SBA) of a serial concentration (10

50 μg/ml) to isolate and enrich FNRBCs by using N-acetylgalactosamine (GalNAc)-coated chamber slides. Results The best working concentration of SBA was 20 μg/ml

which resulted in most effectively isolating and enriching FNRBCs in umbilical cord blood

when the coating concentration of GalNAc was 100 μg/ml. The ratio of white blood cells to nucleated cells were the lowest value (27.7%)

and that of FNRBCs to nucleated cells were the highest value (3.7%). Conclusion GalNAc-specific agglutinin could be used to effectively isolate and enrich FNRBCs.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Non-invasive prenatal diagnosis for homozygous α⁰-thalassemia based on paternal different SNP with allele-specific real-time fluorescence PCR: establishment and application

Related Author

刘彦慧

吴亚敏

石少权

娄季武

马秋林

何怡

徐婉芳

林洋洋

Related Institution

No data

⁰