Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease

武强; 李露斯; 范文辉; 宋敏; 杨忠

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Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease

更新时间：2026-01-09

- Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease
- Journal of Army Medical University Vol. 29, Issue 10, Pages: 915-918(2007)
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  CLC： R329.2;R651.11;R749.160.5
- Published：2007
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武强,李露斯,范文辉,宋敏,杨忠.小鼠胚胎神经干细胞海马移植对APP/PS1双转基因AD小鼠的治疗作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):915-918.

　WU Qiang,LI Lu-si,FAN Wen-hui,SONG Min,YANG Zhong.Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):915-918.
武强,李露斯,范文辉,宋敏,杨忠.小鼠胚胎神经干细胞海马移植对APP/PS1双转基因AD小鼠的治疗作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):915-918. DOI：

　WU Qiang,LI Lu-si,FAN Wen-hui,SONG Min,YANG Zhong.Therapeutic effect of mouse embryonic neural stem cells replacement into hippocampus of APP/PS1 double transgenic mice of Alzheimer disease[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):915-918. DOI：

摘要

目的在APP/PS1双转基因阿尔茨海默病（Alzheimer disease， AD）小鼠观察神经干细胞（nerual stem cells， NSCs）移植后细胞的存活、迁移以及对小鼠记忆功能的影响。方法增强型绿色荧光蛋白（enhanced green fluorescent protein， EGFP）质粒转染培养胚胎NSCs，小鼠海马内移植，水迷宫实验检测小鼠认知功能。结果 EGFP转染NSCs海马内移植2个月后可观察到GFP阳性细胞，大部分分布在针道附近，部分向同侧皮层迁移，亦有部分通过胼胝体向对侧大脑迁移，同时小量细胞发出类似于神经元的长突起。AD小鼠的记忆功能明显改善。结论胚胎NSCs海马内移植后能存活、迁移并分化为神经组织细胞，并能显著改善APP/PS1双转基因AD小鼠的认知功能障碍。

Abstract

Objective To observe the survival

migration and effect of mouse embryonic neural stem cells (NSCs) after NSCs transplantation into the hippocampus of APP/PS1 double transgenic mice of Alzheimer disease (AD). Methods EGFP gene was transfered into NSCs by Nucleofector

. The memory of mice was evaluated by Morris water maze test after NSCs transplantation into the hippocampus. Results Two months after NSCs transplantation

most of the GFP positive cells were found near the pin hole

and some migrated into the ipsilateral cortex as well as opposite side cortex through corpus callosum. Long processes were observed

in a few positive cells. Hidden platform test showed the mean latency in transgenic mice transplanted with NSCs was markedly shortened. Conclusion Transplanted NSCs can survive

migrate and differentiate into nerve cells in the hippocampus of APP/PS1 double transgenic mice. The memory impairment of APP/PS1 double transgenic mice of Alzheimer disease was markedly relieved.

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