Preparation and identification of mimovirus tumor vaccine based on survivin

杨曌; 牛微; 姜曼; 吴玉章; 赵建平; 王莉

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Preparation and identification of mimovirus tumor vaccine based on survivin

更新时间：2026-01-09

- Preparation and identification of mimovirus tumor vaccine based on survivin
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 705-707(2007)
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  CLC： R392-33;R392.11;R73-3
- Published：2007
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杨曌,牛微,姜曼,吴玉章,赵建平,王莉.基于survivin的“模拟病毒”瘤苗的制备和鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):705-707.

　YANG Zhao,NIU Wei,JIANG Man,WU Yu-zhang,ZHAO Jian-ping,WANG Li.Preparation and identification of mimovirus tumor vaccine based on survivin[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):705-707.
杨曌,牛微,姜曼,吴玉章,赵建平,王莉.基于survivin的“模拟病毒”瘤苗的制备和鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):705-707. DOI：

　YANG Zhao,NIU Wei,JIANG Man,WU Yu-zhang,ZHAO Jian-ping,WANG Li.Preparation and identification of mimovirus tumor vaccine based on survivin[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):705-707. DOI：

摘要

目的制备、鉴定基于肿瘤抗原survivin的“模拟病毒”瘤苗。方法在特定的条件下将阳离子肽和DNA制备成模拟病毒颗粒样的结构，并以扫描电镜、DNA阻滞试验、DNaseⅠ保护试验和免疫印迹（Western blotting）对该疫苗进行制备鉴定。结果在NaCl浓度为87.5 mmol/L，电荷比为2的制备条件下，该疫苗能形成颗粒，并可有效介导瘤苗所携带基因的表达。结论该研究成功制备了基于肿瘤抗原survivin的“模拟病毒”瘤苗，为新型的肿瘤疫苗的设计奠定了基础。

Abstract

Objective To prepare and identify the mimovirus tumor vaccine based on survivin. Methods Under specific condition

peptide and DNA were condensed into like-virus particle

and the preparing parameters were optimized by assays of DNA gel retardation

DNase Ⅰ protection and identified by Western blotting. Antigen presentation was also studied by RMA-S/Kd cells. Results The vaccine was condensed into homogeneous particles at charge ratio of 2 in 87.5 mmol/L NaCl concentration

which could effectively mediate expression of the genes carried by vaccine particles. Conclusion The mimovirus tumor vaccine based on survivin was prepared successfully

providing a good foundation for the design of new tumor vaccine.

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