Effects and related mechanism of low-dosed methyl-mercury chloride in promoting apoptosis of intestine epithelial cells of fetal mice

林雪梅; 张海英; 姜蓉; 汪维伟

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Effects and related mechanism of low-dosed methyl-mercury chloride in promoting apoptosis of intestine epithelial cells of fetal mice

更新时间：2026-01-09

- Effects and related mechanism of low-dosed methyl-mercury chloride in promoting apoptosis of intestine epithelial cells of fetal mice
- Journal of Army Medical University Vol. 29, Issue 5, Pages: 410-412(2007)
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  CLC： R321.5;R322.45;R995
- Published：2007
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林雪梅,张海英,姜蓉,汪维伟.低剂量甲基汞促进鼠胚肠上皮细胞凋亡及相关机制的体内实验[J].陆军军医大学学报(原第三军医大学学报),2007,29(05):410-412.

　LIN Xue-mei,ZHANG Hai-ying,JIANG Rong,WANG Wei-wei.Effects and related mechanism of low-dosed methyl-mercury chloride in promoting apoptosis of intestine epithelial cells of fetal mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(05):410-412.
林雪梅,张海英,姜蓉,汪维伟.低剂量甲基汞促进鼠胚肠上皮细胞凋亡及相关机制的体内实验[J].陆军军医大学学报(原第三军医大学学报),2007,29(05):410-412. DOI：

　LIN Xue-mei,ZHANG Hai-ying,JIANG Rong,WANG Wei-wei.Effects and related mechanism of low-dosed methyl-mercury chloride in promoting apoptosis of intestine epithelial cells of fetal mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(05):410-412. DOI：

摘要

目的探讨低剂量氯化甲基汞（methyl-mercury chloride

MMC）对小鼠胚胎肠上皮细胞凋亡的影响及相关机制。方法给E12.5d、E13.5d孕鼠一次性腹腔注射MMC液（0、1、 2、 4 mg/kg），48 h后取十二指肠、结肠。所获标本均制成石蜡切片，部分经HE染色，体视学法计数肠上皮凋亡小体；其余切片用于免疫组化染色，半定量分析凋亡相关蛋白Bcl-2、Bax及即刻早期基因c-fos的表达。结果 ①各实验组凋亡小体数均高于对照组(P0.05)，与MMC剂量呈正相关(P0.01)。②Bcl-2在各组上皮中的表达均低于对照组(P0.05)，与MMC的剂量呈负相关(P0.01)；而Bax及c-fos的表达均强于对照组(P0.05)，与MMC的剂量呈正相关(P0.01)。结论低剂量MMC在体内可促进胎鼠肠上皮细胞凋亡，可能是一种通过下调Bcl-2表达，上调Bax及c-fos表达的机制。

Abstract

Objective To study the effects and related mechanism of low-dosed methyl-mercury chloride (MMC) on the epithelial apoptosis of fetal mice intestine. Methods Pregnant mice on E12.5 d and E13.5 d were injected with different doses of MMC (0

4 mg/kg) intraperitoneally. After 48 h

their duodenum and colon were dissected out and sliced into the paraffin sections. Some sections were stained with HE to count the numbers of apoptotic bodies (NAB) with stereological method. The others were stained with the immunohistochemical method to observe the expression of apoptosis-associated proteins Bcl-2

Bax and immediate early gene c-fos. Results ①In all experimental groups

the NAB in the epithelium were higher than that of control groups (P0.05)

and there was a positive correlation between the doses of MMC and the NAB (P0.01). ②In all experimental groups

the expression of Bcl-2 in the epithelium was lower than that of control groups (P0.05)

and there was a negative correlation between the doses of MMC and the expression of Bcl-2 (P0.01). However the expression of Bax and c-fos were higher than that of in control groups (P0.05). There was a positive correlation between the doses of MMC and the expression of these proteins (P0.01). Conclusion In vivo

MMC can increase the epithelial apoptosis in fetal mouse intestine

by the mechanism of restraining the expression of Bcl-2 and promoting the expression of Bax and c-fos.

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Related Institution

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Immunology, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Laboratory Animal Science， College of Basic Medical Sciences, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Pharmaceutical Analysis, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Orthopedics, Dongnan Hospital

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