Combining hepatocyte transplantation intraperitoneally and augmenter of liver regeneration for acute hepatic failure in rats

王志毅; 张艳; 石小枫; 刘杞

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Combining hepatocyte transplantation intraperitoneally and augmenter of liver regeneration for acute hepatic failure in rats

更新时间：2026-01-09

- Combining hepatocyte transplantation intraperitoneally and augmenter of liver regeneration for acute hepatic failure in rats
- Journal of Army Medical University Vol. 29, Issue 10, Pages: 899-902(2007)
- 作者机构：
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  CLC： R575.305;R657.3;R977.6
- Published：2007
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王志毅,张艳,石小枫,刘杞.肝细胞移植联合肝再生增强因子治疗大鼠急性肝功能衰竭的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):899-902.

　WANG Zhi-yi,ZHANG Yan,SHI Xiao-feng,LIU Qi.Combining hepatocyte transplantation intraperitoneally and augmenter of liver regeneration for acute hepatic failure in rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):899-902.
王志毅,张艳,石小枫,刘杞.肝细胞移植联合肝再生增强因子治疗大鼠急性肝功能衰竭的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):899-902. DOI：

　WANG Zhi-yi,ZHANG Yan,SHI Xiao-feng,LIU Qi.Combining hepatocyte transplantation intraperitoneally and augmenter of liver regeneration for acute hepatic failure in rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):899-902. DOI：

摘要

目的探讨同种异体肝细胞腹腔移植联合肝再生增强因子（augmenter of liver regenration

ALR）对D-氨基半乳糖(D-gal)诱导的急性肝功能衰竭大鼠的治疗作用。方法采用D-gal诱导大鼠急性肝功能衰竭，诱导后24 h，59只大鼠随机数字表法分成4组。Ⅰ组15只：腹腔注射生理盐水；Ⅱ组15只：经腹腔移植2×10

肝细胞

以后腹腔注射生理盐水；Ⅲ组15只：经腹腔移植2×10

肝细胞，同时腹腔注射ALR 50 μg·kg

-1

·d

-1

；Ⅳ组14只：腹腔注射ALR50 μg·kg

-1

·d

-1

。观察大鼠的存活率、肝脏功能、移植肝细胞存活情况、病理变化。结果 Ⅱ组、Ⅲ组大鼠存活率(46.7%、66.7%)显著高于Ⅰ组(0.0%)(P=0.006；P=0.0002)，Ⅲ组(66.7%)与Ⅳ组(14.3%)有显著差异(P=0.008)，其他组间无明显差异(P0.05)。移植后第1、2天Ⅱ组腹水AST高于Ⅲ组（P=0.001）

Ⅱ组第1、2天腹水ALT高于Ⅳ组，Ⅱ组、Ⅲ组第1、2天腹水TBil高于Ⅰ组（P0.05）。Ⅲ组肝脏病理改变轻于其他组。Ⅲ组大鼠腹腔移植的肝细胞存活数多于Ⅱ组。结论同种异体肝细胞经腹腔移植联合ALR可明显改善D-gal诱导的肝衰竭大鼠的存活率，促进移植肝细胞存活和增殖，促进肝组织及功能恢复。

Abstract

Objective To investigate the effect of allogeneic hepatocyte transplantation (HcT) plus augmenter of liver regenration (ALR) intraperitoneally on acute hepatic failure (AHF) induced by D-galactosamine (D-gal) in rats. Methods AHF was induced by D-gal in SD rats. HcT was received 24 h after intoxication

and the AHF rats were divided into four groups randomly. Group Ⅰ receiv

ed normal saline (NS) intraperitoneally. Group Ⅱ received 2×10

hepatocytes intraperitoneally and daily NS. Group Ⅲ received 2×10

hepatocytes intraperitoneally and daily intraperitoneal injection of ALR (50 μg·kg

-1

·d

-1

) simultaneously. Group Ⅳ received daily intraperitoneal injection of ALR (50 μg·kg

-1

·d

-1

). The survival rate of rats

the survival rate of transplanted hepatocytes

liver function and liver histology of all rats were observed. Results Survival rate of Group Ⅱ

Group Ⅲ was higher than that of GroupⅠ(46.7%

66.7% vs 0.0%

P=0.006

P=0.000 2)

that of Group Ⅲ was higher than Group Ⅳ (66.7% vs 14.3%

P=0.008)

without significant difference among other groups. Ascitic aspartate aminotransferase (AST) of Group Ⅱ was higher than that of Group Ⅲ on day 1

2 after transplantation (P=0.001). Ascitic alanine aminotransferase (ALT) of Group Ⅱ was higher than that of Group Ⅳ on day 1

2 after transplantation (P0.05). Ascitic total bilirubin of Group Ⅱ

Group Ⅲ was higher than that of GroupⅠ (P0.05). Hepatic histologic changes of Group Ⅲ was improved more than other groups. The function and histologic changes of transplanted hepatocytes were obviously improved in Group Ⅲ as compared with that of Group Ⅱ. Conclusion After HcT plus ALR intraperitoneally

the survival rate of AHF rats intoxicated with D-gal was increased

and their liver function and histologic changes were also improved. ALR improved the survival and proliferation of transplanted hepatocytes.

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