Mechanism of suppression on proliferation of human colon carcinoma cell line HT29 by oxaliplatin

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Mechanism of suppression on proliferation of human colon carcinoma cell line HT29 by oxaliplatin

更新时间：2026-01-09

- Mechanism of suppression on proliferation of human colon carcinoma cell line HT29 by oxaliplatin
- Journal of Army Medical University Vol. 29, Issue 7, Pages: 606-609(2007)
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  CLC： R73-36;R735.35;R979.1
- Published：2007
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向征,张才全,汤为学.奥沙利铂抗人结肠癌细胞株HT29增殖及其机制的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):606-609.

　XIANG Zheng,ZHANG Cai-quan,TANG Wei-xue.Mechanism of suppression on proliferation of human colon carcinoma cell line HT29 by oxaliplatin[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):606-609.
向征,张才全,汤为学.奥沙利铂抗人结肠癌细胞株HT29增殖及其机制的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):606-609. DOI：

　XIANG Zheng,ZHANG Cai-quan,TANG Wei-xue.Mechanism of suppression on proliferation of human colon carcinoma cell line HT29 by oxaliplatin[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):606-609. DOI：

摘要

目的观察抗肿瘤药物奥沙利铂对人结肠癌细胞株HT29体外增殖的影响，初步探讨其作用机制。方法采用人结肠癌细胞株HT29作为研究对象，MTT法检测不同浓度和作用时间的奥沙利铂对细胞增殖的抑制作用；透射电镜观察细胞形态和超微结构的改变；流式细胞仪分析细胞周期的分布及凋亡的情况；免疫细胞化学法检测细胞周期蛋白及凋亡相关基因蛋白的表达。结果奥沙利铂对HT29细胞的增殖有抑制作用而且呈剂量和时间依赖性。奥沙利铂作用72 h后，电镜可见凋亡细胞及凋亡小体；流式细胞仪检测发现细胞阻滞于G

/M期，G

、S期细胞减少及出现凋亡峰；免疫细胞化学显示Bax和Fas表达增强，CyclinB

、Bcl-2、PCNA表达减弱。结论奥沙利铂对HT29结肠癌细胞的增殖有抑制作用，其作用机制与细胞周期阻滞及诱导细胞凋亡有关。

Abstract

Objective To observe the effects of oxaliplatin on proliferation in human colon carcinoma cell lines HT29 in vitro and investigate the mechanism. Methods The inhibition of proliferation in HT29 cell was estimated by MTT test. Morphologic changes were observed under electron microscope. Distribution of cell cycle and apoptosis was analyzed by using flow cytometry. The expression of cell cycle protein and apoptosis-associated gene protein was detected with immunohistochemical technique. Results Oxaliplatin could inhibit the proliferation of HT29 cells and the inhibition depended on the exposure dose and time. When treated with ox

aliplatin for 72 h

apoptotic cells and apoptotic body could be found under the transmission electronic microscope. The analysis of cell cycle indicated that oxaliplatin blocked cells at G

/M phases and cells of G

and S phase reduced. The expression of Fas and Bax was up-regulated; Bcl-2

CyclinB

and PCNA down-regulated. Conclusion Oxaliplatin could inhibit proliferation of the colon carcinoma cell lines HT29. The mechanism of inhibited proliferation is related to the induced apoptosis and the blocked cells.

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