Componential analysis of acellular scaffold of spinal cord

郭树章; 任先军; 蒋涛; 吴波

您当前的位置：

首页 >

文章列表页 >

Componential analysis of acellular scaffold of spinal cord

更新时间：2026-01-09

- Componential analysis of acellular scaffold of spinal cord
- Journal of Army Medical University Vol. 29, Issue 13, Pages: 1313-1315(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R318.08;R322.81
- Published：2007
- 稿件说明：
移动端阅览
郭树章,任先军,蒋涛,吴波.脱细胞脊髓支架的成分分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(13):1313-1315.

　GUO Shu-zhang,REN Xian-jun,JIANG Tao,WU Bo.Componential analysis of acellular scaffold of spinal cord[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1313-1315.
郭树章,任先军,蒋涛,吴波.脱细胞脊髓支架的成分分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(13):1313-1315. DOI：

　GUO Shu-zhang,REN Xian-jun,JIANG Tao,WU Bo.Componential analysis of acellular scaffold of spinal cord[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1313-1315. DOI：

摘要

目的探讨同种异体脱细胞脊髓支架的制备方法并分析其可能含有的有效成分。方法采用冻融+化学萃取方法制备脱细胞脊髓支架，光镜及扫描电镜观察其结构特征；用免疫组织化学法分析脱细胞支架的主要成分。结果正常的大鼠脊髓组织经脱细胞处理后，清除了所有的细胞成分及髓鞘和轴突，扫描电镜显示保存了细胞外基质成分构成了三维支架结构。成分分析结果显示，存留的支架结构中含有层黏连蛋白（laminin

LN）、纤连蛋白（fibronectin

FN）、Ⅳ型胶原等诱导和促进神经再生、促进细胞黏附和增殖的重要成分。结论脱细胞异体脊髓具有三维支架结构，含有诱导和促进神经再生的相关蛋白，有利于受损神经的再生及移植细胞的存活和增殖。

Abstract

Objective To prepare the acellular scaffold of spinal cord and analyze its component. Methods The acellular scaffold was prepared with the freeze thawing and chemical extraction

its structure was observed by HE and SEM

its component was analyzed by immunohistochemistry. Results The cells

myelin sheath and axon of nerve fibers in the rat spinal cord were eliminated

but three-dimensional supports of extracellular matrix were reserved. The analytical results showed the component of the acellular spinal cord contain laminin

fibronectin and type Ⅳ collagen—the necessary components to facilitate and induce the regeneration of the injured nerves and enhance the adhesion and proliferation of cells. Conclusion The acellular spinal cord has three dimensional structure and contains several proteins related to the regeneration of the injured nerves and promotion of the survival and proliferation of cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

S-Equol improves type 2 diabetes mellitus combined with nonalcoholic fatty liver disease by regulating SREBP pathway and PPARγ in rats

Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis

Alterations of glutamine transport in intestinal epithelial cells and brush border membrane in rats after severe burn injury

GPR48 and TLR4 expression regulates progression of steroid-induced avascular necrosis of the femoral head in rats

Effect of genipin cross-linking on biological characteristics of rat acellular spinal cord

Related Author

Zhe XU

Xiangmin NI

Shuo LI

Guiming ZHANG

Hanqiang CUI

Jian WANG

杨冠

吴东叶

Related Institution

Department of Nutrition, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

⁰