Effect of STAT3 siRNA on proliferation and apoptosis of gastric cancer cells in vitro

张伟; 吴强; 谭德明

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Effect of STAT3 siRNA on proliferation and apoptosis of gastric cancer cells in vitro

更新时间：2026-01-09

- Effect of STAT3 siRNA on proliferation and apoptosis of gastric cancer cells in vitro
- Journal of Army Medical University Vol. 29, Issue 7, Pages: 603-605(2007)
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  CLC： R394-33;R73-362;R735.2
- Published：2007
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张伟,吴强,谭德明.STAT3小干扰RNA的构建及其对胃癌细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):603-605.

　ZHANG Wei,WU Qiang,TAN De-ming.Effect of STAT3 siRNA on proliferation and apoptosis of gastric cancer cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):603-605.
张伟,吴强,谭德明.STAT3小干扰RNA的构建及其对胃癌细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):603-605. DOI：

　ZHANG Wei,WU Qiang,TAN De-ming.Effect of STAT3 siRNA on proliferation and apoptosis of gastric cancer cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):603-605. DOI：

摘要

目的以信号转导与转录激活因子3 (signal transducer and activator of transcription 3，STAT3 )为靶基因，设计并构建短发夹状小干扰RNA表达载体，探讨STAT3小干扰RNA表达载体对胃癌细胞株SGC7901增殖和凋亡的影响。方法以PAVU6＋27质粒为载体，构建STAT3的小干扰RNA表达载体，并将其转染胃癌SGC7901细胞，G418筛选后经反转录聚合酶链反应和Western blot法检测STAT3基因和蛋白表达的改变，MTT法和

H-TdR掺入率检测细胞增殖和DNA合成情况，原位凋亡检测细胞凋亡率，流式细胞术检测抗细胞凋亡因子Bcl-2的表达。结果酶切鉴定和测序分析表明靶向STAT3的RNA干扰重组体构建成功；重组体转染SGC7901后，STAT3的mRNA和蛋白水平较空载体转染组显著下降；MTT比色A值和

H-TdR掺入率均明显低于对照组（P0.05），凋亡率升高(P0.01)，Bcl-2表达下降。结论 STAT3小干扰RNA表达载体可能是抑制人胃癌细胞增殖和促进凋亡的有效手段。

Abstract

Objective To design and construct a vector of signal transducer and activator of transcription 3 (STAT3) small interfering RNA (siRNA)，and to investigate the effect of the recombinant plasmid on the proliferation and apoptosis of gastric cancer cell SGC7901. Methods The PAVU6＋27-STAT3 siRNA expression vector was constructed， then transfected into the cultured gastric cancer cells SGC7901 by DOTAP method. STAT3 mRNA and protein in SGC7901 were determined by RT-PCR and Western blotting respectively. Cell proliferation was tested by MTT and

H-TdR incorporation. Cell apoptosis percentage and bcl-2 expression were observed by TUNEL and flow cytometry respectively. Results The PAVU6＋27-STAT3 siRNA expression vector was successfully constructed. In the transfected SGC7901 cells

cell proliferation

H-TdR incorporation and bcl-2 expression were decreased

and cell apoptosis percentage was increased. Conclusion PAVU6＋27-STAT3 siRNA expression vector may be an efficient method to inhibit the proliferation and promote the apoptosis of gastric cancer cells.

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