Objective    To explore the relationship between HBV load and the contents of serologic markers.      Methods    Time-resolved immunofluorometric assay (TRIFA) was used to detect the contents of HBV immunological markers and real-time fluorescent quantitative PCR (FQ-PCR) was used to measure the HBV DNA of 1 486 clinical serum samples. The data was analyzed by SPSS11.0.      Results    The HBsAg contents were extremely different in the same or different models of serologic markers. The HBV copies of HBeAg(+) patients were significantly more than HBeAb(+) and HBeAg(-)HBeAb(-) patients (P0.01)

but without statistical significance between HBeAb(+) and HBeAg(-)HBeAb(-) patients (P0.05). The contents of both HBsAg and HBeAg were positively correlated with the load of HBV DNA (r=0.383

0.635

P=0.01)

while HBeAb negatively correlated to it (r=-0.563

P=0.01). Direct correlation existed between the contents of HBsAg and HbeAg (r=0.466

P=0.01) while inverse correlation was observed between the contents of HBsAg and HbeAb (r=-0.524

P=0.01).      Conclusion    A certain correlation exists between the contents of serologic markers and DNA of hepatitis B but the great variance exists among individuals. Combining FQ-PCR with TRIFA method is profitable for judging the state of HBV viral replication roundly and provides scientific and reliable evidences for clinical decisions.