Effect of nitric oxide on myocardial cell injury after hypoxia in rats

张铭; 黄跃生; 张琼

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Effect of nitric oxide on myocardial cell injury after hypoxia in rats

更新时间：2026-01-09

- Effect of nitric oxide on myocardial cell injury after hypoxia in rats
- Journal of Army Medical University Vol. 29, Issue 11, Pages: 1017-1019(2007)
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  CLC： R322.11;R329.24;R364.4
- Published：2007
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张铭,黄跃生,张琼.不同浓度NO对缺氧心肌细胞损害的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1017-1019.

　ZHANG Ming,HUANG Yue-sheng,ZHANG Qiong.Effect of nitric oxide on myocardial cell injury after hypoxia in rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1017-1019.
张铭,黄跃生,张琼.不同浓度NO对缺氧心肌细胞损害的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1017-1019. DOI：

　ZHANG Ming,HUANG Yue-sheng,ZHANG Qiong.Effect of nitric oxide on myocardial cell injury after hypoxia in rats[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1017-1019. DOI：

摘要

目的利用外源性一氧化氮(nitric oxide，NO)供体S-亚硝基谷胱甘肽（S-nitrosoglutathione

GSNO）对比观察不同浓度NO对缺氧心肌细胞活力的影响。方法培养4~6 d心肌细胞分为常氧组(N)、缺氧组(H)和缺氧+GSNO组(H+G)；H+G组依照加入GSNO浓度的不同又分为3组：缺氧+GSNO 30 μmol/L组(H+G30)、缺氧+GSNO 200 μmol/L组(H+G200)、缺氧+GSNO 4 000 μmol/L组(H+G4000)。分别观察其对缺氧(1%O

、5%CO

和 94%N

) 4 h心肌细胞损害的影响。结果缺氧引起培养心肌细胞上清LDH活性增加，细胞存活率（MTT法）降低，H+G30组和H+G4000组损伤加重，H+G200组损伤减轻。结论一定浓度范围内的NO具有细胞保护效应。

Abstract

Objective To clarify the effect of NO on hypoxic myocardium in rats. Methods Myocardial cells from neonatal Wistar rats aged 1-2 days cultured for 4-6 days

then pretreated with GSNO (30

200

4 000 μmol/L) were exposed to normoxia (N) or hypoxia (H)(1%O

5%CO

and 94%N

) for 4 h. The NO content and LDH in the supernatant were detected. Results NO of 1.43

7.2 and 70 μmol/L were detected respectively in the supernatant of hypoxic myocardial cells treated by GSNO (30

200 and 4 000 μmol/L). Compared with normoxic group

LDH activity increased and myocardial cell activity decreased markedly in H+G30 and H+G4000 groups but les

s injury was observed in H+G200 group. Conclusion NO of certain concentration can markedly abate myocardial cell injury induced by hypoxia.

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Related Institution

Institute of Immunology, College of Basic Medical Science，Army Medical University （Third Military Medical University）

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Department of Pathophysiology， Faculty of High Altitude Military Medicine, Second Affiliated Hospital， Army Medical University （Third Military Medical University）

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