Regulation of histone acetylation on cell cycle and p53,p21<sup>WAF1</sup> genes expression in malignant transformation of IEC-6

王燕; 郭乔楠; 章波; 许雪青; 白云

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Regulation of histone acetylation on cell cycle and p53,p21^WAF1 genes expression in malignant transformation of IEC-6

更新时间：2026-01-09

- Regulation of histone acetylation on cell cycle and p53,p21^WAF1 genes expression in malignant transformation of IEC-6
- Journal of Army Medical University Vol. 29, Issue 4, Pages: 300-303(2007)
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  CLC： R394.3;R730.2;R735.3
- Published：2007
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王燕,郭乔楠,章波,许雪青,白云.组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21WAF1基因表达的调控[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):300-303.

　WANG Yan,GUO Qiao-nan,ZHANG Bo,XU Xue-qing,BAI Yun.Regulation of histone acetylation on cell cycle and p53,p21WAF1 genes expression in malignant transformation of IEC-6[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):300-303.
王燕,郭乔楠,章波,许雪青,白云.组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21WAF1基因表达的调控[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):300-303. DOI：

　WANG Yan,GUO Qiao-nan,ZHANG Bo,XU Xue-qing,BAI Yun.Regulation of histone acetylation on cell cycle and p53,p21WAF1 genes expression in malignant transformation of IEC-6[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):300-303. DOI：

摘要

目的分析和探讨IEC-6恶性转化细胞不同时期组蛋白乙酰化修饰对肿瘤发生相关基因表达的影响。方法采用我们已建立的3个转化期(化学致癌剂MNNG和诱导剂PMA共同作用6、12、24次) 的IEC-6转化细胞进行培养，提取不同转化时期的IEC-6转化细胞的细胞核蛋白，采用免疫印迹检测H3乙酰化水平；并用ChIP及RT-PCR检测组蛋白H3乙酰化对p21

WAF1

表达的影响。结果 24次IEC-6恶性转化细胞组蛋白H3乙酰化水平高于6次IEC-6转化细胞，而组蛋白H3去乙酰化水平低于6次IEC-6转化细胞；同时发现p21

WAF1

的启动子区PCR产物减少。在6、12次IEC-6转化细胞中均有较弱的p53、p21

WAF1

表达；在24次IEC-6恶性转化细胞中仍可检测到较弱的p53表达，但p21

WAF1

表达缺如。结论 IEC-6恶性转化细胞过程中，组蛋白H3乙酰化水平增高，导致p21

WAF1

基因表达受抑，近而可能影响转化细胞的细胞周期。

Abstract

Objective To investigate the effects of histone acetylation on the expression of p53 and p21

WAF1

genes in malignant transformation of IEC-6. Methods The malignant transformation of IEC-6 was carried out by self-established three transition phases in which chemical cancerogenic agent MNNG and derivative PMA were used to the target cells for 6

and 24 times. The transformants were cultured and the neucleoprotein of IEC-6 was extracted during every transition phase. The cell cycle distribution wa

s studied by flow cytometry. The level of acetylated histones in chromatin associated with the p53 and p21

WAF1

was examined by chromatin immunoprecipitation (ChIP) assay. The expression of p53 and p21

WAF1

genes mRNA was detected by RT-PCR. Results Acetylation level of histone H3 in IEC-6 was higher after malignant transformation for 24 times than 6 times. Malignant transformation induced an accumulation of acetylated histone H3 in chromatin associated with p21

WAF1

gene. Expression of p53 and p21

WAF1

was detected weakly in 6 and 12 times malignant transformation of IEC-6. There was no expression of p21

WAF1

in malignant transformation of IEC-6 for 24 times. Conclusion In the malignant transformation of IEC-6 cell

the expression of p21

WAF1

and p53 genes is regulated by histone acetylation. The enhanced acetylation of histone H3 will restrain the expression of p21

WAF1

gene and further may affect the cell cycle of transformants.

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Regulation of histone acetylation on cell cycle and p53,p21WAF1 genes expression in malignant transformation of IEC-6

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摘要

Abstract

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Keywords

references

Regulation of histone acetylation on cell cycle and p53,p21^WAF1 genes expression in malignant transformation of IEC-6