Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro

孙兴旺; 曹灵; 于国华; 唐学清; 郭庆喜; 张弦; 王巍; 许凯

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Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro

更新时间：2026-01-09

- Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro
- Journal of Army Medical University Vol. 29, Issue 7, Pages: 585-587(2007)
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  CLC： R285.5;R329.28;R692.05
- Published：2007
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孙兴旺,曹灵,于国华,唐学清,郭庆喜,张弦,王巍,许凯.丹参酮Ⅱ-A磺酸钠对纤维化人肾间质成纤维细胞体外增殖及cyclin E蛋白表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):585-587.

　SUN Xing-wang,CAO Ling,YU Guo-hua,TANG Xue-qing,GUO Qing-xi,ZHANG Xuan,WANG Wei,XU Kai.Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):585-587.
孙兴旺,曹灵,于国华,唐学清,郭庆喜,张弦,王巍,许凯.丹参酮Ⅱ-A磺酸钠对纤维化人肾间质成纤维细胞体外增殖及cyclin E蛋白表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(07):585-587. DOI：

　SUN Xing-wang,CAO Ling,YU Guo-hua,TANG Xue-qing,GUO Qing-xi,ZHANG Xuan,WANG Wei,XU Kai.Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(07):585-587. DOI：

摘要

目的研究丹参酮Ⅱ-A磺酸钠（sodium tanshinone Ⅱ-A sulfonate，DS-201）对人肾间质纤维化来源的成纤维细胞(human renal interstitial fibroblasts，hRIFs)体外增殖及细胞周期素E（cyclin E）基因表达的影响，探讨该药治疗肾间质纤维化的作用机制。方法体外培养并鉴定hRIFs；用四甲基偶氮唑（MTT）法检测对照组与不同DS-201浓度组hRIFs的增殖活性；用免疫细胞化学S-P法和图像分析技术检测对照组与不同DS-201浓度组hRIFs cyclin E基因的表达。结果随药物浓度的升高和作用时间的延长，抑制率逐渐升高，抑制作用逐渐增强，呈剂量依赖性和时间依赖性。用药组阳性细胞的平均光密度值在第3、5、7、9天显著低于对照组（P0.05，P0.01），第1天用药各组与对照组之间无统计学差异（P0.05）。结论 ① DS-201对hRIFs体外增殖有显著抑制作用，可能是其治疗肾间质纤维化的机制之一。② DS-201抑制hRIFs体外增殖可能是通过抑制细胞中cyclin E基因的表达，阻滞细胞通过G

/S关卡，延长细胞周期实现的。

Abstract

Objective To study the effect of sodium tanshinone Ⅱ-A sulfonate (DS-201)on the proliferation of human renal interstitial fibroblasts (hRIFs) and the expression of cyclin E. Methods After hRIFs were cultured and identified

MTT was used to detect the cells at passage 3-4 in present or absent of DS-201at different concentrations. The expression of cyclin E was analyzed by immunocytochemistry and the image-analytical technique. Results With the increase of the dr

ug concentration and prolongation of action time

the inhibitory ratio was increased in a dose- and time-dependent manner. The expression of cyclin E was stronger in drug groups than in control group on the 3rd

5th

7th and 9th day (P0.05，P0.01). Conclusion DS-201 significantly inhibits the hRIFs in vitro，which may be one of its mechanisms to suppress renal interstitial fibrosis. The mechanism of this inhibition may be due to that sodium tanshinone Ⅱ-A sulfonate inhibits the expression of cyclin E

thus blocks the G

/S point and extents the cell cycle.

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