Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library

黄英; 蔡雪飞; 张君; 黄爱龙

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Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library

更新时间：2026-01-09

- Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library
- Journal of Army Medical University Vol. 29, Issue 10, Pages: 876-878(2007)
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  CLC： R341;R373.21;R394-33
- Published：2007
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黄英,蔡雪飞,张君,黄爱龙.用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):876-878.

　HUANG Ying,CAI Xue-fei,ZHANG Jun,HUANG Ai-long.Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):876-878.
黄英,蔡雪飞,张君,黄爱龙.用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):876-878. DOI：

　HUANG Ying,CAI Xue-fei,ZHANG Jun,HUANG Ai-long.Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):876-878. DOI：

摘要

目的用T7噬菌体筛选系统筛选HCV核心蛋白的相互作用蛋白。方法应用T7噬菌体展示技术，以通过原核表达的方式得到HCV的核心蛋白作为靶分子，对噬菌体人肝细胞cDNA文库进行筛选，对筛选到的克隆进行DNA序列分析及同源性搜索。结果经鉴定得到1个阳性克隆，确定了能与HCV核心蛋白相互作用的蛋白为：Smad interacting-protein 1（SIP1）。结论 T7噬菌体展示筛选系统是筛选相互作用蛋白的一种简单、快速和有效的手段，筛选到的相互作用蛋白为进一步探讨核心蛋白的致病、致癌机制提供重要依据。

Abstract

Objective To screen and identify proteins that interact with the hepatitis C virus core protein by means of T7-phage display system. Methods The hepatitis C virus core protein was expressed by prokaryotic expression and used as selected molecule to biopan the T7 human liver cDNA library. The selected positive clones were identified by DNA sequence and analyzed with BLAST program in GenBank. Results After BLAST in all positive clones

one protein--Smad interacting protein 1 (SIP1) was found to interact with the hepatitis C virus core protein. Conclusion T7-phage display system is a convenient

rapid and effective method for screening interacting proteins.

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