WANG Gui-bo,WANG Xiao-qin,LUO Gao-xing,HE Wei-feng,CHEN Xi-wei,WU Jun,BIAN Xiu-wu.Design of a multifunction medical experiment platform and its temperature regulation in cell in vitro culture[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1289-1292.
WANG Gui-bo,WANG Xiao-qin,LUO Gao-xing,HE Wei-feng,CHEN Xi-wei,WU Jun,BIAN Xiu-wu.Design of a multifunction medical experiment platform and its temperature regulation in cell in vitro culture[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1289-1292.DOI:
Design of a multifunction medical experiment platform and its temperature regulation in cell in vitro culture
Objective To design a multifunction platform for medical experiments. Methods Sensors and controllers of temperature
humidity
oxygen and carbon dioxide concentration were used to make the platform for medical experiments. The relative criteria of this machine were tested. The cell lines 293 and PK15 were used to test the potential usage of the platform. Results The temperature
humidity
oxygen concentration
carbon dioxide concentration in this medical experimental platform were well controlled. The range of temperature control was from -20 to +60 ℃
the range of carbon dioxide concentration control from 0% to 20%
the range of oxygen concentration control from 0% to 100%. The range of humidity control was from the humidity at room temperature to 98%. The morphological changes of 293 and PK15 cell lines could be found when temperature went down as designed. Conclusion The temperature
oxygen concentration
carbon dioxide concentration and humidity of storage chamber can be intellectually controlled. This apparatus can be used for graft storage-related test.