Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils

郭刚; 童文德; 邹全明; 吴超; 刘开云

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Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils

更新时间：2026-01-09

- Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils
- Journal of Army Medical University Vol. 29, Issue 11, Pages: 1006-1009(2007)
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  CLC： R378.99;R394-33;R394.3
- Published：2007
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郭刚,童文德,邹全明,吴超,刘开云.HP0318基因在幽门螺杆菌适应性定植中的作用研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1006-1009.

　GUO Gang,TONG Wen-de,ZOU Quan-ming,WU Chao,LIU Kai-yun.Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1006-1009.
郭刚,童文德,邹全明,吴超,刘开云.HP0318基因在幽门螺杆菌适应性定植中的作用研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1006-1009. DOI：

　GUO Gang,TONG Wen-de,ZOU Quan-ming,WU Chao,LIU Kai-yun.Role of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1006-1009. DOI：

摘要

目的通过构建幽门螺杆菌(Hp) HP0318缺失突变株，初步探明该基因在Hp适应性定植中的作用。方法通过基因克隆和重组技术在HP0318基因上下游片段之间连入氯霉素抗性基因（catGC）作为筛选标记，并克隆到质粒pBluescript SK中构建成缺失突变的打靶载体，将其转化沙鼠适应株M

，经过同源重组，对出发菌的HP0318基因进行置换突变，然后用经氯霉素抗性筛选的突变菌株感染蒙古沙鼠，观察其与未突变的M

出发菌在定植能力和致病性上的差异。结果经PCR鉴定成功构建1株缺失HP0318基因的Hp菌株。突变株与非突变株对蒙古沙鼠的感染率相差显著(P0.05)，2个菌株在胃内的定植密度有显著性差异(P0.05)，突变株定植密度有约5倍的减少。结论 HP0318基因在Hp的沙鼠适应性定植过程中发挥了重要的作用。

Abstract

Objective To construct and identify a deficiency mutant of H. pylori strains without HP0318 gene. Methods Two cloning PCR fragments containing two-end regions of HP0318 and a selectable chloramphenicol resistance marker between them were engineered into pBluescript plasmid. Then the HP0318-deleted derivative plasmid was electrotransformed into adaptive strain M

and constructed a HP0318-negative mutant of H. pylori via homologous recombination resulting in allelic exchange. M

and the mutant were inoculated to Mongolian gerbils

respectively. The level of colonization and pathological change were assessed at 4 weeks after infection. Results A

n HP0318 negative mutant of H. pylori strain has been constructed successfully. The infectious rate and colonization levels of the HP0318 mutant in Mongolian gerbils were significantly lower as compared with M

(P0.05). Conclusion These results demonstrate the importance of HP0318 gene for adaptive colonization of H. pylori in Mongolian gerbils.

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