Clone and eukaryotic expression of human cannabinoid receptor 2 gene

王颖莹; 陈纯海; 裴莉萍; 张伟; Norbert E. Kaminski

您当前的位置：

首页 >

文章列表页 >

Clone and eukaryotic expression of human cannabinoid receptor 2 gene

更新时间：2026-01-09

- Clone and eukaryotic expression of human cannabinoid receptor 2 gene
- Journal of Army Medical University Vol. 29, Issue 11, Pages: 1003-1005(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.2;R394-33
- Published：2007
- 稿件说明：
移动端阅览
王颖莹,陈纯海,裴莉萍,张伟,Norbert E Kaminski.2型大麻受体的克隆及真核表达体系的构建[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1003-1005.

　WANG Ying-ying,CHEN Chun-hai,PEI Li-ping,ZHANG Wei,Norbert E. Kaminski.Clone and eukaryotic expression of human cannabinoid receptor 2 gene[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1003-1005.
王颖莹,陈纯海,裴莉萍,张伟,Norbert E Kaminski.2型大麻受体的克隆及真核表达体系的构建[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1003-1005. DOI：

　WANG Ying-ying,CHEN Chun-hai,PEI Li-ping,ZHANG Wei,Norbert E. Kaminski.Clone and eukaryotic expression of human cannabinoid receptor 2 gene[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):1003-1005. DOI：

摘要

目的克隆人2型大麻受体（hCB2）基因，并构建相应的稳定表达细胞株。方法利用人T淋巴细胞株HPB-ALL的总RNA，以RT-PCR及酶切、连接等分子生物学方法克隆hCB2基因于质粒载体pFlag-CMV-3内，以HEK293细胞构建稳定表达细胞株，以Western blot方法利用蛋白标记Flag的单克隆抗体（Anti-Flag）和CB2多克隆抗体（Anti-CB2）同时检测质粒的表达。结果酶切及测序结果表明hCB2基因克隆成功，Western blot检测结果表明Anti-Flag和Anti-CB2都能检测Flag-hCB2蛋白。结论成功克隆hCB2基因，并获得稳定表达细胞株，同时证实Anti-CB2多克隆抗体有效。

Abstract

Objective To clone human cannabinoid receptor 2 gene and establish stable cell line which constantly expresses hCB2 protein. Methods Full length of hCB2 cDNA was obtained by RT-PCR with total RNA isolated from human T cell line HPB-ALL

and then constructed into plasmid vector pFlag-CMV-3. The stable cell line was established with HEK293 cells. The expression of Flag-CB2 was analyzed by Western blotting with monoclonal Anti-Flag and multiclonal Anti-CB2. Results pFLag-hCB2 was confirmed containing proper hCB2 gene with diagnostic digestion and sequencing

and the expression products were detected by Anti-Flag and Anti-CB2 parallelly. Conclusion Gene of hCB2 has been cloned and constantly expressed in HEK293 cells. The multiclonal Anti-CB2 is proved efficient in detecting CB2 protein epitope.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Construction of eukaryotic expression of CIB vectors and its expression in NIH3T3 cells

Construction of eukaryotic expression vector pcDNA3.1-RI and its effect on human umbilical vein endothelial cells

Eukaryotic expression and identification of recombinant shuttle plasmid for co-expression of human granulysin active peptide and murine IL-12

Construction of recombinant vector MHC-Ⅰ-fluorescin tetramer and its eukaryotic expression

Expression of pcDNA3-TLR9/GFP in HEK293T cells

Related Author

王晓辉

袁碧波

杨金开

吕同德

惠斌

蔡文琴

惠玲

陈俊霞

Related Institution

No data

⁰