Screening the interaction proteins of MRP2 by yeast two-hybrid system

刘涛; 金超智; 张翠莉; 陈慧; 储著朗; 王英杰; 杨晓明

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Screening the interaction proteins of MRP2 by yeast two-hybrid system

更新时间：2026-01-09

- Screening the interaction proteins of MRP2 by yeast two-hybrid system
- Journal of Army Medical University Vol. 29, Issue 4, Pages: 296-299(2007)
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  CLC： Q503;Q591.2
- Published：2007
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刘涛,金超智,张翠莉,陈慧,储著朗,王英杰,杨晓明.应用酵母双杂交系统筛选MRP2胞内区相互作用蛋白[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):296-299.

　LIU Tao,JIN Chao-zhi,ZHANG Cui-li,CHEN Hui,CHU Zhu-lang,WANG Ying-jie,YANG Xiao-ming.Screening the interaction proteins of MRP2 by yeast two-hybrid system[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):296-299.
刘涛,金超智,张翠莉,陈慧,储著朗,王英杰,杨晓明.应用酵母双杂交系统筛选MRP2胞内区相互作用蛋白[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):296-299. DOI：

　LIU Tao,JIN Chao-zhi,ZHANG Cui-li,CHEN Hui,CHU Zhu-lang,WANG Ying-jie,YANG Xiao-ming.Screening the interaction proteins of MRP2 by yeast two-hybrid system[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):296-299. DOI：

摘要

目的应用酵母双杂交技术研究与多药耐药蛋白MRP2发生相互作用的蛋白。方法应用酵母双杂交系统，以人MRP2胞内区C末端220个氨基酸为诱饵，筛选成人肝cDNA文库，寻找能与之相互作用的蛋白。利用生物信息学分析，一对一酵母回复性杂交等提供的信息，筛除假阳性。结果经过酵母双杂交共获得162个克隆，经过验证分析，最终确定3个无重复阳性克隆。结论获得的3个基因编码的蛋白可能揭示MRP2新的作用机制。

Abstract

Objective To study interaction proteins of MRP2 by yeast two-hybrid system. Methods Using the 220 C-terminal amino acids of MRP2 as bait

a human liver cDNA library was screened and the proteins interacting with MRP2 were searched. The false clones were eliminated by bioinformatic methods and one to one yeast two-hybrid system. Results Three true positive clones from yeast clones were obtained after verification. Conclusion The encoding proteins we obtained may play important roles in finding the new function of MRP2.

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