In vitro anti-tumor immune response induced by dendritic cells transfected with DeltaNp73α recombinant adenovirus

胡义杰; 范士志; 蒋耀光; 李志平; 陈建明; 何勇

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In vitro anti-tumor immune response induced by dendritic cells transfected with DeltaNp73α recombinant adenovirus

更新时间：2026-01-09

- In vitro anti-tumor immune response induced by dendritic cells transfected with DeltaNp73α recombinant adenovirus
- Journal of Army Medical University Vol. 29, Issue 9, Pages: 763-766(2007)
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  CLC： R563.104
- Published：2007
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胡义杰,范士志,蒋耀光,李志平,陈建明,何勇.人DeltaNp73α基因修饰的树突状细胞抗瘤免疫学效应研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(09):763-766.

　HU Yi-jie,FAN Shi-zhi,JIANG Yao-guang,LI Zhi-ping,CHEN Jian-ming,HE Yong.In vitro anti-tumor immune response induced by dendritic cells transfected with DeltaNp73α recombinant adenovirus[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(09):763-766.
胡义杰,范士志,蒋耀光,李志平,陈建明,何勇.人DeltaNp73α基因修饰的树突状细胞抗瘤免疫学效应研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(09):763-766. DOI：

　HU Yi-jie,FAN Shi-zhi,JIANG Yao-guang,LI Zhi-ping,CHEN Jian-ming,HE Yong.In vitro anti-tumor immune response induced by dendritic cells transfected with DeltaNp73α recombinant adenovirus[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(09):763-766. DOI：

摘要

目的研究DeltaNp73α重组腺病毒转染树突状细胞诱导的特异性免疫抗瘤作用。方法人脐静脉血经梯度离心及IL-4、GM-CSF诱导培养树突状细胞；DeltaNp73α重组腺病毒增强离心法转染树突状细胞，观察DeltaNp73α对树突状细胞活化、凋亡的影响；同时检测其所诱导的DeltaNp73α特异性细胞毒性T淋巴细胞对不同DeltaNp73α表达水平肿瘤细胞的细胞毒性作用。结果 Western blot证实重组腺病毒转染树突状细胞后DeltaNp73α的有效表达；DeltaNp73α修饰的树突状细胞凋亡率显著低于对照组；DeltaNp73α修饰树突状细胞诱导的细胞毒性T细胞可以有效的杀伤DeltaNp73α高表达的A549/DeltaNp73α细胞、K-562细胞；且这种免疫抗瘤作用可能与靶肿瘤细胞DeltaNp73α水平相关。结论 DeltaNp73α修饰的树突状细胞，其凋亡率降低；并可以有效诱导针对DeltaNp73α高表达肿瘤细胞的特异免疫抗瘤作用。

Abstract

Objective To investigate the effects of dendritic cells infected with adenovirus vector encoding DeltaNp73α on breaking the immune tolerance and inducing immunity against DeltaNp73α-overexpressing tumor. Methods Immature dendritic cells generated in the presence of interleukin-4 and granulocyte/macrophage colony-stimulating factor from human umbilical cord blood were transfected with recombinant adenovirus vector encoding full-length human DeltaNp73α cDNA by centrifugal force method. Viability and activation status of DCs after transfection were assessed by flow cytometric analysis

and induction of DeltaNp73α-specific CTL response was also evaluated by the cytotoxic assay against DeltaNp73α-overexpressing human tumor cells. Results DeltaNp73α expression in the transfected DCs was confirmed by Western blotting. DeltaNp73α enhanced DCs survival. A significant DeltaNp73α-specific CTL-stimulatory ability were observed in the DeltaNp73α-overexpressing cell lines

A549/DeltaNp73α and K-562. Conclusion DeltaNp73α enhances the survival of dendritic cells. Adenovirus vector-mediated DeltaNp73α-transfected DCs can effectively induce strong antigen-specific T cell response.

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