Research on immunogenicity of MSCs after osteoinduction

郭书权; 谢肇; 吴雪晖; 许建中; 梁文杰; 罗飞

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Research on immunogenicity of MSCs after osteoinduction

更新时间：2026-01-09

- Research on immunogenicity of MSCs after osteoinduction
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 663-665(2007)
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  CLC： R322.71;R329.21;R392.1
- Published：2007
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郭书权,谢肇,吴雪晖,许建中,梁文杰,罗飞.间充质干细胞成骨诱导后免疫原性的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):663-665.

　GUO Shu-quan,XIE Zhao,WU Xue-hui,XU Jian-zhong,LIANG Wen-jie,LUO Fei.Research on immunogenicity of MSCs after osteoinduction[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):663-665.
郭书权,谢肇,吴雪晖,许建中,梁文杰,罗飞.间充质干细胞成骨诱导后免疫原性的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):663-665. DOI：

　GUO Shu-quan,XIE Zhao,WU Xue-hui,XU Jian-zhong,LIANG Wen-jie,LUO Fei.Research on immunogenicity of MSCs after osteoinduction[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):663-665. DOI：

摘要

目的研究间充质干细胞(mesenchymal stem cells

MSCs)成骨诱导后的免疫原性。方法小型猪骨髓MSCs体外分离培养，成骨诱导1、7、14、21 d后，行流式检测MHC-Ⅱ，单向混合淋巴细胞培养（mixed lymphocyte culture

MLC）测定刺激指数(stimulation index

SI)，并测定混和培养上清IL-2和IL-10的变化。结果 MSCs诱导后1、7、14、21 d均未检测到MHC-Ⅱ表达，MLC未测到细胞明显增殖，上清IL-2和IL-10无显著变化（P0.05）。结论 MSCs体外成骨诱导后的免疫原性低。

Abstract

Objective To study the the immunogenicity of MSCs after differentiation into osteocytes. Methods MSCs isolated from pig bone barrow were cultured and induced into differentiate into osteocytes. Before induction and on day 1

14 and 21 after induction

MHC-Ⅱ was detected with flow cytometer (FCM)

stimulation index was examined by One-way MLC

and IL-2 and IL-10 levels in supernatant was determined. Results MSCs after osteoinduction did not express MHC-Ⅱ

and there were no significant changes of stimulation index

IL-2 and IL-10 levels. Conclusion The immunogenicity of MSCs after differentiation into bone is low.

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