Objective    To construct the eukaryotic expression vector of human HNF1α gene and to observe its expression in vitro.     Methods    Total RNA from HepG2 cell was used to acquire human HNF1α cDNA by RT-PCR. Then HNF1α cDNA was cloned into T-A vector. The recombined vector confirmed by sequencing was digested by HindⅢ/EcoRⅠ to obtain HNF1α cDNA fragment

then the fragment was subcloned into multiple sites of pcDNA3.1(+) vector. The identified recombinant plasmid was transfected into HepG2 cell with liposome. The expressions of HNF1α in the cell were determined with RT-PCR and Western blot.     Results    The recombinant pcDNA3.1(+)-HNF1α plasmid was constructed successfully and the overexpression of HNF1α gene could be detected in the transfected HepG2 cell.     Conclusion    The recombinant expression pcDNA3.1(+)-HNF1α vector is successfully constructed and can be expressed in vitro.