Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice

李军; 粟永萍; 王军平; 艾国平; 邹仲敏

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Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice

更新时间：2026-01-09

- Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice
- Journal of Army Medical University Vol. 29, Issue 8, Pages: 655-657(2007)
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  CLC： R-332;R45;R644.02
- Published：2007
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李军,粟永萍,王军平,艾国平,邹仲敏.颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):655-657.

　LI Jun,SU Yong-ping,WANG Jun-ping,AI Guo-ping,ZOU Zhong-min.Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):655-657.
李军,粟永萍,王军平,艾国平,邹仲敏.颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):655-657. DOI：

　LI Jun,SU Yong-ping,WANG Jun-ping,AI Guo-ping,ZOU Zhong-min.Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):655-657. DOI：

摘要

目的观察颈交感神经阻滞（sympathetic block

SB）对烧伤小鼠早期炎症介质核因子-κB（NF-κB）及其辅活化子的影响，初步探讨SB在严重创伤中治疗作用的分子机制。方法将雄性昆明小鼠分为正常对照组、烧伤对照组、LSB组（烧伤后使用利多卡因进行SB治疗）、RSB组（烧伤后使用罗哌卡因进行SB治疗），每组5只。烧伤为TBSA 15%~20%Ⅲ度。烧伤后6 h采用Western blotting测定肝组织NF-κB P65、类固醇受体辅活化子-3（SRC-3）的蛋白表达及核转位。结果烧伤对照组肝组织NF-κB P65、SRC-3蛋白表达水平及核转位显著增加；与烧伤对照组相比，LSB组NF-κB P65蛋白表达无显著变化，SRC-3蛋白表达及NF-κB P65、SRC-3核转位显著下调；RSB组NF-κB P65、SRC-3蛋白表达及核转位均显著低于烧伤对照组，且比LSB组下调更明显。结论烧伤早期（6 h）NF-κB及其辅活化子SRC-3蛋白表达水平及核转位显著上调，SB在严重创伤中的治疗作用可能是通过抑制NF-κB功能活性，降低炎症反应程度而实现的。

Abstract

Objective To observe the effect of cervical sympathetic block (SB) on NF-κB and its coactivator during the early stage in burned mice. Methods Kunming male mice were randomly divided into normal control

burn control

LSB group (treated immediately by SB with Lidocaine after burn injury) and RSB group (treated immediately by SB with Ropivacaine after burn injury). Third degree burn injury involving 15%-20% of TBSA was produced. At 6 h after burn

the expression and nuclear translocation of hepatic NF-κB P65 and steroid receptor coactivator-3 (SRC-3) were detected by Western blotting. Results After burn injury

the expression and nuclear translocation of hepatic NF-κB P65 and SRC-3 were markedly elevated as compared with normal control group. The level of SRC-3 and nuclear translocation of NF-κB P65 and SRC-3 in LSB group were significantly downregulated compared with that of burn control group

but the NF-κB P65 was no significant difference with that of burn control group. The expression and nuclear translocation of NF-κB P65 and SRC-3 in RSB group were obviously lower than burn control group and LSB group. Conclusion The expression and nuclear translocation of hepatic NF-κB P65 and SRC-3 are markedly elevated during the early stage of burn injury. SB can exert supression on the function of NF-κB and reduce the degree of systemic inflammatory response

which may be the mechanisms for therapeutic effect of SB on severe trauma.

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