Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD<sub>2</sub>

郝磊; 邹仲敏; 王军平; 董世武; 邓均; 闫国和; 王连友; 宁宇; 刘登群; 罗成基; 粟永萍

您当前的位置：

首页 >

文章列表页 >

Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD₂

更新时间：2026-01-09

- Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD₂
- Journal of Army Medical University Vol. 29, Issue 10, Pages: 859-862(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R331.22;R394-33;R394.2
- Published：2007
- 稿件说明：
移动端阅览
郝磊,邹仲敏,王军平,董世武,邓均,闫国和,王连友,宁宇,刘登群,罗成基,粟永萍.hPDGF-A/hBD2双基因共表达腺病毒载体的构建及表达[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):859-862.

　HAO Lei,ZOU Zhong-min,WANG Jun-ping,DONG Shi-wu,DENG Jun,YAN Guo-he,WANG Lian-you,NING Yu,LIU Deng-qun,LUO Cheng-ji,SU Yong-ping.Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD2[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):859-862.
郝磊,邹仲敏,王军平,董世武,邓均,闫国和,王连友,宁宇,刘登群,罗成基,粟永萍.hPDGF-A/hBD2双基因共表达腺病毒载体的构建及表达[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):859-862. DOI：

　HAO Lei,ZOU Zhong-min,WANG Jun-ping,DONG Shi-wu,DENG Jun,YAN Guo-he,WANG Lian-you,NING Yu,LIU Deng-qun,LUO Cheng-ji,SU Yong-ping.Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD2[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):859-862. DOI：

摘要

目的制备人血小板源性生长因子-A(human platelet-derived growth factor A

hPDGF-A) 和人β防御素2（human beta defensins

hBD

）双基因共表达腺病毒载体并观察其在大鼠骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells

BMSCs)中的表达。方法将hPDGF-A和hBD

通过内部核糖体插入位点(internal ribosome entry site

IRES) 连接后

以同源重组的形式插入腺病毒表达载体

由人胚肾293 细胞包装，获得有感染能力的重组腺病毒颗粒。重组病毒感染BMSCs后，用RT-PCR检测重组腺病毒的表达。结果 ①成功构建穿梭质粒pAdTrack-hPDGF-A-IRES

-hBD

。②成功构建重组腺病毒质粒pAdeasy-hPDGF-A-IRES

-hBD

。③293细胞包装获得有感染能力的重组腺病毒。④转染的BMSCs高表达hPDGF-A和hBD

。结论构建了hPDGF-A/hBD

双基因共表达腺病毒载体

证实其转染BMSC后的表达。

Abstract

Objective To further determine their possible synergistic effect on accelerating wound healing

adenovirus vector containing recombinant human hPDGF-A and hBD

genes was constructed and the expression of exogenous genes in transformed mesenchymal stem cells derived from rat bone marrow was observed. Methods By putting IRES in the middle of hPDGF-A and hBD

these two genes were expected to be expressed individually. The

shuttle vector was named as pAdTrack-hPDGF-A-IRES

-hBD

which homologously recombinated with Adeasy-1 in BJ5183 cells and formed the mammalian expression vector pAdeasy-hPDGF-A-IRES

-hBD

. Furthermore

the recombinant vector was packaged in 293 cells into infectious recombinant adenovirus

which were used to infect BMSCs. The expression of hPDGF-A and hBD

in BMSCs was detected by RT-PCR. Results We successfully constructed recombinant adenovirus vector that simultaneously expressed hPDGF-A and hBD

. The expressions of hPDGF-A and hBD

were confirmed by RT-PCR on transformed BMSCs. Conclusion The established BMSCs that overexpressed hPDGF-A and hBD

provide a new strategy of combining cell therapy and gene therapy to promote wound healing

especially the chronic one.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Concern over global outbreak of acute severe hepatitis of unknown etiology in children

Expression and mechanism of rat hyperplasia suppressor gene suppressing growth of rat C6 glioma cells

Construction and expression of recombinant adenovirus vector of erythropoietin in rat spiral ganglion neurons

Trichostatin A enhances transfection efficiency of oncolytic adenovirus in vitro through up-regulating CAR in human esophageal cancer cell line EC9706

Construction of recombinant adenovirus with siANGPTL4 gene and its inhibitive effect on MG63 cell proliferation

Related Author

Songtao ZHAO

Qing MAO

沈冰

赵巍

方志豪

杨强

郭晖

蒋树财

Related Institution

Institute of Infectious Diseases, Chongqing Key Laboratory of Infectious Diseases Research, First Affiliated Hospital, Army Medical University (Third Military Medical University)

⁰

Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD2

DOI：

摘要

Abstract

关键词

Keywords

references

Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD₂