Objective     To construct the recombinant adenovirus vector containing mouse PPARδ

and infect the INS-1 cells.      Methods     The pCDNA3.0-mPPARδ plasmid was digested with KpnⅠ and EcoRⅤ to obtain full-length coding sequence of mPPARδ

then the mPPARδ cDNA was ligated to the pAdtrack-CMV vector. The plasmid of pAdTrack-mPPARδ was linearized with PmeⅠ

and the fragment was reclaimed and transformed into BJ5183 which contains pAdeasy. After being screened

the extracted plasmid of positive bacteria linearized with PacⅠ w

as transfected into HEK293 cells with lipofectamine

TM

2000 and was identified by the green fluorescence protein (GFP). The harvested virus was amplified in HEK293 cells and infected INS-1 cells. After infection

the expression of PPARδ was proved by GFP expression and Western blotting.      Results     The recombinant adenovirus vector containing mouse PPARδ was constructed successfully and the titer was 1.5×10

10

pfu/ml. The PPARδ expression in INS-1 cells infected by the virus was much higher than the controls.      Conclusion     The constructed recombinant adenovirus vector containing mouse PPARδ provides a potent tool to investigate its biological function in pancreatic islets and other tissues.