Effect of human ovarian cancer cell apoptosis induced by arsenic trioxide on telomerase activity

宁燕; 米粲

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Effect of human ovarian cancer cell apoptosis induced by arsenic trioxide on telomerase activity

更新时间：2026-01-09

- Effect of human ovarian cancer cell apoptosis induced by arsenic trioxide on telomerase activity
- Journal of Army Medical University Vol. 29, Issue 1, Pages: 62-64(2007)
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  CLC： R73-354;R737-31;R979.19
- Published：2007
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宁燕,米粲.三氧化二砷诱导人卵巢癌细胞凋亡对端粒酶活性的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):62-64.

　NING Yan,MI Can.Effect of human ovarian cancer cell apoptosis induced by arsenic trioxide on telomerase activity[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):62-64.
宁燕,米粲.三氧化二砷诱导人卵巢癌细胞凋亡对端粒酶活性的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):62-64. DOI：

　NING Yan,MI Can.Effect of human ovarian cancer cell apoptosis induced by arsenic trioxide on telomerase activity[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):62-64. DOI：

摘要

目的探讨As

诱导细胞凋亡时对其端粒酶活性的影响及其作用机制。方法采用光镜、电镜， PCR-ELISA，RT-PCR等技术，检测细胞凋亡，端粒酶活性及其催化亚单位的表达。结果 As

可诱导卵巢癌细胞凋亡，但存在作用浓度，细胞株类型差异性;端粒酶活性随药物作用时间和浓度的增加而明显减弱，同时伴随hTERT mRNA表达水平的下降。结论端粒酶活性和hTERT mRNA表达在As

诱导SKOV

、3AO细胞凋亡过程中共同发挥作用，两者有密切关系，是诱导细胞凋亡的分子机制之一。

Abstract

Objective To investicate the effect of apoptosis of human ovarian cancer cell line SKOV

and 3AO exposed to arsenic trioxide on telomerase activity and its mechanisms. Methods The human ovarian cancer cell line SKOV3 and 3OA were treated with arsenic trioxide of different concentration for 12

72 h. Cell morphology

PCR-ELISA

RT-PCR were adopted to detect the cell apoptosis and telomerase activity and the expression of human telomerase catalytic subunit (hTERT). Results Arsenic trixide could induce apoptisis of ovarian cancer cell lines

but there exists difference in drug concentration

type of cell line. A dose and time-dependent decline of telomerase activity after SKOV

and

3AO cells exposed to arsenic trixide

meanwhile hTERT mRNA was down-regulated. Conclusion Telomerase activity and hTERT mRNA expression have close relationship and play an important role in arsenic trioxide inducing SKOV

and 3AO cells apoptosis.

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米粲

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Related Institution

Hunan Provincial Key Laboratory of Vascular Biology and Translational Medicine， Hunan University of Chinese Medicine

Department of Burn Wounds and Plastic Surgery， First Affiliated Hospital of Hunan University of Chinese Medicine

Air Force Clinical College, Anhui Medical University

Department of Gastroenterology, Air Force Medical Center of PLA

Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

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