Expression of NF-κB P65 in mouse to rat cardiac xenograft model

杨光伦; 姚榛祥; 黄平; 涂刚

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Expression of NF-κB P65 in mouse to rat cardiac xenograft model

更新时间：2026-01-09

- Expression of NF-κB P65 in mouse to rat cardiac xenograft model
- Journal of Army Medical University Vol. 29, Issue 1, Pages: 51-52(2007)
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  CLC： R-332;R392.4;R654.2
- Published：2007
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杨光伦,姚榛祥,黄平,涂刚.NF-κB P65在大鼠异种心脏移植后心肌中的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):51-52.

　YANG Guang-lun,YAO Zhen-xiang,HUANG Ping,TU Gang.Expression of NF-κB P65 in mouse to rat cardiac xenograft model[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):51-52.
杨光伦,姚榛祥,黄平,涂刚.NF-κB P65在大鼠异种心脏移植后心肌中的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):51-52. DOI：

　YANG Guang-lun,YAO Zhen-xiang,HUANG Ping,TU Gang.Expression of NF-κB P65 in mouse to rat cardiac xenograft model[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):51-52. DOI：

摘要

目的复制小鼠→大鼠异种心脏移植模型，探讨核转录因子κB （nuclear factor-κB ，NF-κB） P65蛋白在异种移植心肌中的表达及NF-κB的DNA结合活性。方法动物随机数字法分成2组：A组(对照组)，n=6；B组(移植组)，n=6。采用免疫印迹杂交法（Western blot）检测对照组及移植组心肌组织NF-κB P65表达，凝胶电泳迁移率实验检测NF-κB DNA结合活性。结果移植组的NF-κB P65蛋白表达和NF-κB DNA结合活性均显著高于对照组(P0.05)。结论 NF-κB 的激活可能在大鼠异种移植心脏排斥反应中起着重要作用。

Abstract

Objective To reproduce mouse to rat heart transplantation model and determine the expression and DNA binding activity of NF-κB P65 in xenograft heart. Methods Six NIH mice were used as heart donors and six Wistar rats as heart recipients. After the transplantation

the transplanted hearts without any drug intervention were prepared for experiment after cardiac arrest. The hearts from another six NIH mice served as normal controls. The expression of NF-κB P65 protein and NF-κB P65 DNA binding activity in xenograft heart were determined by Western blotting and electrophoretic mobility shift assay (EMSA). Results The expression level of NF-κB P65 and NF-κB P65 DNA binding activity in transplanted hearts were higher than control hearts (P0.05). Conclusion NF-κB may play an important role in mouse to rat heart transplantation.

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Related Institution

Urinary Nephropathy Center， the Second Affiliated Hospital of Chongqing Medical University

Department of Immunology， College of Basic Medical Sciences

Department of Nephrology and Urology， No. Hospital958， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Military Cognitive Psychology, Faculty of Medical Psychology, Army Medical University (Third Military Medical University)

Henan Key Laboratory of Traditional Chinese Medicine Syndrome and Prescription in Signaling, Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling, Academy of Chinese Medicine, Henan University of Chinese Medicine

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