Effect of sendai virus replication sequence on protein changes in immature dendritic cells

易发平; 张平波; 郭风劲; 马永平; 宋方洲

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Effect of sendai virus replication sequence on protein changes in immature dendritic cells

更新时间：2026-01-09

- Effect of sendai virus replication sequence on protein changes in immature dendritic cells
- Journal of Army Medical University Vol. 29, Issue 1, Pages: 15-17(2007)
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  CLC： R338.12;R373;R394.3
- Published：2007
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易发平,张平波,郭风劲,马永平,宋方洲.仙台病毒复制序列对未成熟树突状细胞蛋白表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):15-17.

　YI Fa-ping,ZHANG Ping-bo,GUO Feng-jin,MA Yong-ping,SONG Fang-zhou.Effect of sendai virus replication sequence on protein changes in immature dendritic cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):15-17.
易发平,张平波,郭风劲,马永平,宋方洲.仙台病毒复制序列对未成熟树突状细胞蛋白表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(01):15-17. DOI：

　YI Fa-ping,ZHANG Ping-bo,GUO Feng-jin,MA Yong-ping,SONG Fang-zhou.Effect of sendai virus replication sequence on protein changes in immature dendritic cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):15-17. DOI：

摘要

目的研究仙台病毒载体复制序列对未成熟树突状细胞（dendritic cells， DC）蛋白质表达的影响，为联合运用二者进行基因治疗提供依据。方法提取去除仙台病毒复制序列载体（Sev/△F）、完全序列载体（SeV）转染未成熟DC的总蛋白，定量。双向凝胶电泳分离蛋白质组分，胶体银染显色，PDQuest进行图像分析后选取差异点，胶内酶解后MALDI-TOF MS进行肽指纹图谱鉴定。结果图像分析结果显示，Sev/△F感染后的DC明显比SeV感染后的DC表达蛋白点多，其中有不少蛋白表达量也明显上调。结论仙台病毒载体感染未成熟DC后引起蛋白表达的减少，这与SeV的复制序列有关。

Abstract

Objective To investigate the protein change in immature dendritic cells (DC) exposed to sendai virus replication sequence. Methods The total cellular proteins expressed in DC infected with Sev/△F or SeV were collected and quantitated

then separated by two-dimensional gel electrophoresis and visualized by silver staining. Digital images were analyzed by PDQuest software. The differentially expressed protein spots were picked

digested in gel and subjected to peptide mass fingerprinting using MALDI-TOF MS. Results Protein spots in 2-DE gel for DC treated by Sev/△F were more than that by SeV

among which a lot of proteins increased. Conclusion It was relevant to the replication sequence that proteins decreased in dendritic cells infected with sendai virus vector.

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