Effect of Shengmai injection on cell apoptosis in rat myocardium after burn

张西联; 黄跃生; 党永明; 张家平; 郑霁; 房亚东; 周军利; 张琼

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Effect of Shengmai injection on cell apoptosis in rat myocardium after burn

更新时间：2026-01-09

- Effect of Shengmai injection on cell apoptosis in rat myocardium after burn
- Journal of Army Medical University Vol. 29, Issue 2, Pages: 94-96(2007)
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  CLC： R289.5;R322.11;R644.02
- Published：2007
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张西联,黄跃生,党永明,张家平,郑霁,房亚东,周军利,张琼.生脉注射液对大鼠烧伤后心肌细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(02):94-96.

　ZHANG Xi-lian,HUANG Yue-sheng,DANG Yong-ming,ZHANG Jia-ping,ZHENG Ji,FANG Ya-dong,ZHOU Jun-li,ZHANG Qiong.Effect of Shengmai injection on cell apoptosis in rat myocardium after burn[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(02):94-96.
张西联,黄跃生,党永明,张家平,郑霁,房亚东,周军利,张琼.生脉注射液对大鼠烧伤后心肌细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(02):94-96. DOI：

　ZHANG Xi-lian,HUANG Yue-sheng,DANG Yong-ming,ZHANG Jia-ping,ZHENG Ji,FANG Ya-dong,ZHOU Jun-li,ZHANG Qiong.Effect of Shengmai injection on cell apoptosis in rat myocardium after burn[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(02):94-96. DOI：

摘要

目的探讨生脉注射液对大鼠烧伤后心肌细胞凋亡的影响。方法 SD(Sprague-Dawley)大鼠72只，随机分成单纯烫伤组（B组，n=36）和烫伤并应用生脉注射液治疗组（S组，n=36），制成30%TBSA Ⅲ度烫伤模型，伤后B组按Parkland公式补液，S组在B组的基础上伤后即刻按4 ml/kg给予生脉注射液，并相应减少补液量，两组均于不同时相点，取左室心肌组织切片，做病理形态学观察，并采用DNA切口末端标记法(TUNEL)和心肌组织caspase-3活性双指标联合检测细胞凋亡。结果病理切片显示S组较B组心肌组织损伤有所减轻；两组心肌细胞TUNEL显示烫伤后6 h呈阳性，伤后12 h达高峰；caspase-3活性变化早于凋亡形态学变化，于伤后3、6 h达峰值; S组较B组在伤后6、12、24 h心肌细胞凋亡指数显著降低（P0.01），左心室心肌组织caspase-3活性在伤后3、6、12 h也显著降低（P0.05）。结论烧伤后早期心肌细胞凋亡参与了烧伤病理损伤过程。早期应用生脉注射液治疗可以有效地减少心肌细胞的凋亡，进而减轻心肌的损伤，起到保护心肌的作用。

Abstract

Objective To investigate the effect of Shengmai injection on cell apoptosis in rat myocardium after burn. Methods Totally 60 adult Sprague-Dawley rats were subject to 30% TBSA full thickenss scald and were randomly divided into two groups: scald group (sodium lactate ringer’s injection was injected after scald according to the formula of Parkland) and Shengmai group (Shengmai injection was applied immediately at dose of 4 ml/kg

and sodium lactate ringer’s injection was reduced correspondingly). Another 12 rats served as control for each group. Left ventricle tissue was prep

ared before scald and at 1

24 h after scald for pathologic examination. The cell apoptosis was determined by terminal deoxynucleotibyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and detecting caspase-3 activity. Results The damage of rat myocardium in Shengmai group was lessened than scald group. The positive staining by TUNEL in rat myocardial cells of the two groups was observed at 6th hour after scald and reached the top level at 12

hour. The change of caspase-3 activity started earlier than apoptosis and reached the peak value at 3

and 6

hour after scald. However

the apoptosis index of myocardium in Shengmai group was more significantly decreased than that of scald group at 6

and 24

hour (P0.01)

so did the change of caspase-3 activity at 3

and 12

hour after scald (P0.05). Conclusion The myocardial apoptosis took part in postburn myocardial injury. The postburn myocardial injury could be lessened effectively by early apply of Shengmai injection.

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