Effect of bFGF in coordination with autografting of bone marrow mesenchymal stem cells on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound

陈涛; 付小兵; 伍津津; 韩冰; 孙同柱

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Effect of bFGF in coordination with autografting of bone marrow mesenchymal stem cells on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound

更新时间：2026-01-09

- Effect of bFGF in coordination with autografting of bone marrow mesenchymal stem cells on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound
- Journal of Army Medical University Vol. 29, Issue 2, Pages: 91-93(2007)
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  CLC： R322.99;R392.11;R641.05
- Published：2007
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陈涛,付小兵,伍津津,韩冰,孙同柱.bFGF与MSCs自体移植对猪皮肤创面PCNA和Ⅷ-R Ag表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(02):91-93.

　CHEN Tao,FU Xiao-bing,WU Jin-jin,HAN Bing,SUN Tong-zhu.Effect of bFGF in coordination with autografting of bone marrow mesenchymal stem cells on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(02):91-93.
陈涛,付小兵,伍津津,韩冰,孙同柱.bFGF与MSCs自体移植对猪皮肤创面PCNA和Ⅷ-R Ag表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(02):91-93. DOI：

　CHEN Tao,FU Xiao-bing,WU Jin-jin,HAN Bing,SUN Tong-zhu.Effect of bFGF in coordination with autografting of bone marrow mesenchymal stem cells on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(02):91-93. DOI：

摘要

目的研究碱性成纤维细胞生长因子（basic fibroblast growth factor， bFGF）和骨髓间充质干细胞(mesenchymal stem cells

MSCs) 自体移植对小型猪全层皮肤缺损创面增殖细胞核抗原(PCNA)和Ⅷ因子相关抗原(Ⅷ-R Ag)表达的影响。方法 6只小型猪背部中线两侧各制备4个面积为2.54 cm

的圆形全层皮肤缺损创面，将创面分为bFGF治疗组、MSCs治疗组、bFGF+MSCs治疗组、生理盐水对照组(共4组，每组12个创面)。分别取不同时段创面组织，进行常规染色，并采用免疫组化方法检测PCNA和Ⅷ-R Ag的表达情况。结果伤后7 d各组创面均形成肉芽组织，以bFGF+MSCs治疗组的血管形成最为丰富。PCNA表达量增加，伤后7 d达到高峰，主要位于肉芽组织中，持续至14 d PCNA表达开始下降，而且在各个时间点，均以bFGF+MSCs治疗组的PCNA阳性细胞百分率为最高(P0.05)。伤后7 d，Ⅷ-R Ag染色的阳性细胞百分率明显高于其余3个组。结论 bFGF协同自体移植的MSCs可使皮肤创面PCNA和Ⅷ-R Ag的表达增加，促进肉芽组织生成，缩短创面愈合时间。

Abstract

Objective To investigate the effect of bFGF and MSCs autografting on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound. Methods Six male minipigs were used to produce 48 full-thickness skin wounds

each wound area about 2.54 cm

on both sides of the back (8 wounds per pig). These 48 wounds were randomly treated with bFGF

MSCs

bFGF+MSCs

and normal saline (12 wounds for each treatment). The tissue from the wounds was harvested o

n day 3

14 after surgery

then used for HE staining and immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and factor Ⅷ related antigen (Ⅷ-R Ag). Results Granulation tissue was presented in all groups on day 3 and 7 after wound

most abundant in the wounds treated with bFGF+MSCs

as well as blood capillary formation. PCNA expression increased after skin injury

reached the peak on day 7

most in the granulation tissue

and lasted to day 14

then began to decrease. On day 3

14 after skin injury

PCNA expression was the most highest in the wounds treated with bFGF+MSCs. Ⅷ-R Ag-positive cells in the wounds treated with bFGF+MSCs were significantly more than that of the other three treatments (P0.05). Conclusion bFGF in coordination with MSCs can promote the expression of PCNA and Ⅷ-R Ag of wounds and accelerate granulation tissue growth.

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State Key Laboratory of Trauma, Burns and Combined Injury, Department of Anti-radiation Medicine, Chongqing Engineering Research Center for Nanomedicine, Institute of Combined Injury, Faculty of Preventive Military Medicine, Army Medical University (Third Military Medical University)

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State Key Laboratory of Trauma, Burns and Combined Injury, Medical Center of Hematology, PLA Center for Hematology, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

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