Association of gut microbiota and their metabolites with clinical phenotypes in perimenopausal women with myasthenia gravis

Wang Rui; Li Yan; Yang Quanjun; Mo Hui

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Association of gut microbiota and their metabolites with clinical phenotypes in perimenopausal women with myasthenia gravis

更新时间：2025-11-18

- Association of gut microbiota and their metabolites with clinical phenotypes in perimenopausal women with myasthenia gravis
- Journal of Chongqing Medical University Pages: 1-8(2025)
- 作者机构：
  
  1.澳门科技大学中医药学院，澳门　999078
  2.贵州中医药大学第二附属医院神经内科，贵阳 550000
  3.上海交通大学医学院附属第六人民医院，上海 200233
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.8
- Received：19 May 2025，
  
  Published Online：18 November 2025，
- 稿件说明：
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王睿,李艳,杨全军等.围绝经期重症肌无力患者的肠道菌群及其代谢产物与临床表型的相关性研究[J].重庆医科大学学报,

Wang Rui,Li Yan,Yang Quanjun,et al.Association of gut microbiota and their metabolites with clinical phenotypes in perimenopausal women with myasthenia gravis[J].Journal of Chongqing Medical University,
王睿,李艳,杨全军等.围绝经期重症肌无力患者的肠道菌群及其代谢产物与临床表型的相关性研究[J].重庆医科大学学报, DOI：10.13406/j.cnki.cyxb..

Wang Rui,Li Yan,Yang Quanjun,et al.Association of gut microbiota and their metabolites with clinical phenotypes in perimenopausal women with myasthenia gravis[J].Journal of Chongqing Medical University, DOI：10.13406/j.cnki.cyxb..

摘要

目的

探讨围绝经期女性重症肌无力（myasthenia gravis，MG）患者的肠道菌群及其代谢产物的变化特征，与临床表型进行关联性分析，探讨潜在的免疫代谢调控机制。

方法

采用病例–对照研究设计，纳入2023年2月至2024年7月贵州中医药大学第二附属医院女性MG患者26例，按生理阶段分为育龄期组（15例）与围绝经期组（11例），另设健康对照组（12例），收集所有研究对象的基本信息及临床表型数据；采集粪便样本，进行16S核糖体DNA（16S ribosomal DNA，16S rDNA）测序和液相色谱–质谱（liquid chromatography–mass spectrometry，LC-MS）代谢组分析，筛选出MG围绝经期有统计学差异的菌群及代谢产物，与MG严重程度评分、睡眠、情绪等临床表型进行Spearman秩相关分析。

结果

与MG育龄期比，围绝经期菌群β多样性差异有统计学意义（

=0.003）。物种差异分析显示，

Aminipila

属、

Anaeroglobus

属、

Hominimerdicola

属等在围绝经期组丰度较高。代谢组学分析提示，MG围绝经期共检测到196种差异代谢产物，主要富集在芳香族氨基酸代谢、谷胱甘肽代谢及三羧酸循环（tricarboxylic acid cycle，TCA cycle）等。Spearman秩相关分析提示：邻羟基苄醇（2-Hydroxybenzyl alcohol）与定量重症肌无力评分（quantitative myasthe

nia gravis score，QMG）显著相关（

=-0.57，

0.05）。

结论

围绝经期MG女性呈现特异性的菌群及代谢谱重塑，可能通过影响芳香族代谢、脂代谢及免疫调节机制，参与临床表型的变化，其为靶向干预围绝经期MG患者肠道微生态提供参考。

Abstract

Objective

To investigate the alterations in gut microbiota and their metabolites in perimenopausal women with myasthenia gravis（MG），as well as their association with clinical phenotypes，and to clarify the underlying immunometabolic regulatory mechanisms.

Methods

A case-control study was conducted among 26 women with MG who were treated in The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from February 2023 to July 2024，and according to their physiological stage，they were divided into reproductive age group with 15 women and perimenopausal group with 11 women； a healthy control group with 12 women was also established. Basic information and clinical phenotypic data were collected，and fecal samples were collected for 16S ribosomal DNA（rDNA） sequencing and iquid chromatography–mass spectrometry（LC-MS）-based metabolomics profiling to identify differentially expressed flora and metabolites in perimenopausal women with MG. A Spearman correlation analysis was used to investigate the correlation of such flora and metabolites with quantitative myasthenia gravis（QMG） score，sleep，and emotion.

Results

There was a significant difference in the beta diversity of gut microbiota between the perimenopausal group and the reproductive age group （

=0.003）. The taxonomic analysis showed a relatively high abundance of

Aminipila

，

Anaeroglobus

，and

Hominimerdicola

in the perimenopausal group. The metabolomics analysis identified 196 differentially expressed metabolites in the perimenopausal group，which were mainly enriched in aromatic amino acid metabolism，glutathione metabolism，and trica

rboxylic acid（TCA） cycle. The Spearman correlation analysis showed that 2-hydroxybenzyl alcohol was significantly correlated with QMG score（

=-0.57，

0.05）.

Conclusion

Perimenopausal women with MG exhibit distinct features of gut microbiota and metabolic profiles，which may be involved in the changes in clinical phenotypes through aromatic compound metabolism，lipid metabolism，and immune modulation. These findings provide a reference for targeted intervention of gut microbiota in perimenopausal women with MG.

关键词

Keywords

references

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